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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation

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Author(s):
Anbumani, Silambarasan [1] ; da Silva, Aldeliane M. [1] ; Carvalho, Isis G. B. [2] ; Fischer, Eduarda Regina [2] ; de Souza e Silva, Mariana [2] ; von Zuben, Antonio Augusto G. [1] ; Carvalho, Hernandes F. [3] ; de Souza, Alessandra A. [2] ; Janissen, Richard [4] ; Cotta, Monica A. [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, BR-13083859 Campinas, SP - Brazil
[2] Citrus Ctr APTA Sylvio Moreira Agron Inst Campina, BR-13490970 Cordeiropolis, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
[4] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands
Total Affiliations: 4
Document type: Journal article
Source: NPJ BIOFILMS AND MICROBIOMES; v. 7, n. 1 DEC 7 2021.
Web of Science Citations: 0
Abstract

The morphological plasticity of bacteria to form filamentous cells commonly represents an adaptive strategy induced by stresses. In contrast, for diverse human and plant pathogens, filamentous cells have been recently observed during biofilm formation, but their functions and triggering mechanisms remain unclear. To experimentally identify the underlying function and hypothesized cell communication triggers of such cell morphogenesis, spatially controlled cell patterning is pivotal. Here, we demonstrate highly selective cell adhesion of the biofilm-forming phytopathogen Xylella fastidiosa to gold-patterned SiO2 substrates with well-defined geometries and dimensions. The consequent control of both cell density and distances between cell clusters demonstrated that filamentous cell formation depends on cell cluster density, and their ability to interconnect neighboring cell clusters is distance-dependent. This process allows the creation of large interconnected cell clusters that form the structural framework for macroscale biofilms. The addition of diffusible signaling molecules from supernatant extracts provides evidence that cell filamentation is induced by quorum sensing. These findings and our innovative platform could facilitate therapeutic developments targeting biofilm formation mechanisms of X. fastidiosa and other pathogens. (AU)

FAPESP's process: 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control
Grantee:Alessandra Alves de Souza
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/07616-3 - New catalysts and directionality control in III-V semiconductor nanowires
Grantee:Mônica Alonso Cotta
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology
Grantee:Hernandes Faustino de Carvalho
Support Opportunities: Research Projects - Thematic Grants