razilian green propolis: A novel tool to improve the cytotoxic and immunomodulatory action of docetaxel on MCF-7 breast cancer cells and on women monocyt

Autor(es)

Cardoso, Eliza de Oliveira ^[1] ; Santiago, Karina Basso ^[1] ; Conti, Bruno Jose ^[1] ; Conte, Fernanda Lopes ^[1] ; Tasca, Karen Ingrid ^[1] ; Romagnoli, Graziela Gorete ^[2] ; Golim, Marjorie de Assis ^[3] ; Rainho, Claudia Aparecida ^[1] ; Bastos, Jairo Kenupp ^[4] ; Sforcin, Jose Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, Campus Botucatu, Sao Paulo - Brazil

^[2] Oeste Paulista Univ UNOESTE, Dept Hlth Sci, Jau - Brazil

^[3] Sao Paulo State Univ UNESP, Botucatu Blood Ctr, Sch Med, Campus Botucatu, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Phytotherapy Research; v. 36, n. 1 DEC 2021.
• Citações Web of Science: 0

Resumo

Docetaxel (DTX) is used against breast cancer despite its side effects such as toxicity and immunosuppression. Here we investigated the cytotoxic and immunomodulatory effects of the ethanol solution extract of propolis (EEP) in combination with DTX on MCF-7 breast cancer cells and on women's monocyte. The cytotoxic potential of EEP + DTX was assessed by MTT assay and the type of tumor cell death was evaluated by flow cytometry. The effects of EEP + DTX on the migration and invasion of MCF-7 cells were analyzed. Cytokine production by monocytes was assessed by ELISA and the expression of cell surface markers was evaluated by flow cytometry. We also assessed the fungicidal activity of monocytes against Candida albicans and the generation of reactive oxygen species (ROS). Finally, the impact of the supernatants of treated monocytes in the viability, migration, and invasiveness of tumor cells was assessed. EEP enhanced the cytotoxicity of DTX alone against MCF-7 cells by inducing necrosis and inhibiting their migratory ability. EEP + DTX exerted no cytotoxic effects on monocytes and stimulated HLA-DR expression, TNF-alpha, and IL-6 production, exerted an immunorestorative action in the fungicidal activity, and reduced the oxidative stress. Our findings have practical implications and reveal new insights for complementary medicine. (AU)

• Processo FAPESP: 16/09986-4 - Ação da própolis associada ao tamoxifeno sobre células de câncer de mama e sobre monócitos humanos: nova abordagem terapêutica?
• Beneficiário: Eliza de Oliveira Cardoso
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático