Green and Chemical Silver Nanoparticles and Pomegranate Formulations to Heal Infected Wounds in Diabetic Rats

Autor(es)

Fernandes Scappaticci, Renan Aparecido ^[1] ; Berretta, Andresa Aparecida ^[2] ; Torres, Elina Cassia ^[2] ; Moreira Buszinski, Andrei Felipe ^[2] ; Fernandes, Gabriela Lopes ^[1] ; dos Reis, Thaila Fernanda ^[1] ; de Souza-Neto, Francisco Nunes ^[3] ; Gorup, Luiz Fernando ^[3] ; de Camargo, Emerson Rodrigues ^[3] ; Barbosa, Debora Barros ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Dent Mat & Prosthodont, Sch Dent, BR-16015050 Aracatuba, SP - Brazil

^[2] Apis Flora Ind & Comercial Ltda, Lab Res Dev & Innovat, BR-14020670 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANTIBIOTICS-BASEL; v. 10, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Infected cutaneous ulcers from diabetic rats with Candida albicans and Streptococcus aureus were treated with spray formulations containing green silver nanoparticles (GS), chemical silver nanoparticles (CS), or pomegranate peel extract (PS). After wound development and infection, the treatments were performed twice per day for 14 days. The wound healing was analyzed on days 2, 7, and 14 through the determination of CFUs, inflammatory infiltrate, angiogenesis, fibroplasia, myeloperoxidase, and collagen determination. Expressive improvement in wound healing was noted using both silver nanoparticles for 7 days. All the treatments were superior to controls and promoted significant S. aureus reduction after 14 days. CS presented better anti-inflammatory results, and GS and CS the highest number of fibroblasts. Despite the techniques' limitations, GS and CS demonstrated considerable potential for managing infected wounds, especially considering no early strategies prior to the drugs, such as the debridement of these wounds, were included. (AU)

• Processo FAPESP: 16/04230-9 - Obtenção de extrato e síntese de nanopartículas de prata a partir de Punica granatum (romã): Caracterização físico-química, determinação da atividade antimicrobiana e potencial cicatrizante
• Beneficiário: Renan Aparecido Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado