ysozyme activity as an indicator of innate immunity of tilapia (Oreochromis niloticus) when challenged with LPS and Streptococcus agalactia

Autor(es)

Biller, Jaqueline Dalbello ^[1] ; Polycarpo, Gustavo do Valle ^[1] ; Moromizato, Basia Schlichting ^[1] ; Diogo Sidekerskis, Ana Paula ^[1] ; da Silva, Thais Daltoso ^[2] ; dos Reis, Ingrid Camargo ^[3] ; Fierro-Castro, Camino ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Tecnol, Dracena, SP - Brazil

^[2] Univ Estadual Paulista, Programa Posgrad Aquicultura, Jaboticabal, SP - Brazil

^[3] Univ Estadual Paulista, Programa Posgrad Ciencia & Tecnol Anim, Dracena, SP - Brazil

^[4] Univ Leon, Fac Ciencias Biol & Ambientales, Dept Biol Mol, Campus Vegazana, Leon - Spain

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE; v. 50, 2021.
• Citações Web of Science: 0

Resumo

The objective of this study was to fully describe the protocol with standardized modifications and evaluate the lysozyme activity, an indicator of innate immunity in tilapia, to compare lipopolysaccharide (LPS) with Streptococcus agalactiae injections. Lysozyme was determined in serum using the turbidimetric method, in which lysozyme activity was evaluated by Micrococcus lysodeikticus lysis, with modifications for microplate assay. The experiment was conducted in a completely randomized design. Juvenile tilapia was divided in the following six treatments: challenged with phosphate buffer PBS (control) and 100, 250, 500, and 600 mu g kg(-1) LPS and S. agalactiae. All treatments were challenged for 72 h and seven days and then sampled to determine lysozyme activity. After 72 h or seven days, concentrations of LPS promoted changes in lysozyme production, either lesser or equal, depending on concentration when compared with fish injected with S. agalactiae. It was possible to standardize the analysis and determine that the treatment with LPS promotes immunomodulation at a concentration of 250 mu g kg(-1) LPS, this response being similar to challenge with S. agalactiae. (AU)

• Processo FAPESP: 12/22016-3 - Estudo do potencial imunoestimulante do b-glucano e seu modo de ação visando novos protocolos de administração em tilápia, Oreochromis niloticus
• Beneficiário: Jaqueline Dalbello Biller Takahashi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores