Erythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

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Chacon, Daisy Sotero ^[1] ; Torres, Taffarel Melo ^[2] ; da Silva, Ivanice Bezerra ^[1] ; de Araujo, Thiago Ferreira ^[1] ; Roque, Alan de Araujo ^[3] ; Senna Domingos Pinheiro, Francisco Ayrton ^[1] ; Selegato, Denise ^[4] ; Pilon, Alan ^[5] ; Santos Reginaldo, Fernanda Priscila ^[1] ; da Costa, Cibele Tesser ^{[6, 7]} ; Vilasboa, Johnatan ^{[6, 7]} ; Teixeira Freire, Rafael ^[8] ; Voigt, Eduardo Luiz ^[9] ; Silveira Zuanazzi, Jose Angelo ^[10] ; Libonati, Renata ^[11] ; Rodrigues, Julia Abrantes ^[11] ; Maia Santos, Filippe Lemos ^[11] ; Scortecci, Katia Castanho ^[9] ; Lopes, Norberto Peporine ^[3] ; Ferreira, Leandro De Santis ^[1] ; dos Santos, Leandro Vieira ^[12] ; Cavalheiro, Alberto Jose ^[13] ; Fett-Neto, Arthur Germano ^{[6, 7]} ; Giordani, Raquel Brandt ^[1]

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^[1] Fed Univ Rio Grande Norte UFRN, Dept Pharm, Natal, RN - Brazil

^[2] Rural Fed Univ Semiarid, Bioinformat Biostat & Comp Biol Nucleus, Mossoro, RN - Brazil

^[3] Inst Sustainable Dev & Environm, Dunas Pk Herbarium, Natal, RN - Brazil

^[4] Univ Florence, Magnet Resonance Ctr CERM, Sesto Fiorentino, FI - Italy

^[5] Univ Sao Paulo FCFRP USP, Fac Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[6] Univ Fed Rio Grande do Sul, Dept Bot, Porto Alegre, RS - Brazil

^[7] Univ Fed Rio Grande do Sul, Plant Physiol Lab, Ctr Biotechnol, Porto Alegre, RS - Brazil

^[8] Barcelona Inst Sci & Technol, Inst Bioengn Catalonia IBEC, Signal & Informat Proc Sensing Syst, Barcelona - Spain

^[9] Univ Fed Rio Grande do Norte, Ctr Biosci, Dept Cell Biol & Genet, Natal, RN - Brazil

^[10] Univ Fed Rio Grande do Sul, Lab Pharmacognosy, Porto Alegre, RS - Brazil

^[11] Univ Fed Rio de Janeiro, Inst Geosci, Dept Meteorol, Rio De Janeiro - Brazil

^[12] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil

^[13] Sao Paulo State Univ UNESP, Chem Inst, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ADVANCED RESEARCH; v. 34, n. SI, p. 123-136, DEC 2021.
• Citações Web of Science: 0

Resumo

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. Erythrina species are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids (BIAs), which can act on several pathology-related biological targets. Objectives: In this sense, in an unprecedented approach used with a non-model Fabaceae species grown in its unique arid natural habitat, a combined transcriptome and metabolome analyses (seeds and leaves) is presented. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a NextSeq 500 platform. Regarding metabolite profiling, the High-resolution Liquid Chromatography was coupled to DAD and a micrOTOF-QII mass spectrometer by using electrospray ionization (ESI) and Time of Flight (TOF) analyzer. The tandem MS/MS data were processed and analyzed through Molecular Networking approach. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids, several of them unique. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: Overall, these results could contribute by indicating potential biotechnological targets for modulation of erythrina alkaloids biosynthesis as well as improve molecular databases with omic data from a non-model medicinal plant, and reveal an interesting chemical diversity of Erythrina BIA harvested in (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Cairo University. (AU)

• Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático