Photodynamic effect of protoporphyrin IX in gliosarcoma 9l/lacZ cell line

Autor(es)

Fontana, Leticia Correa ^[1] ; Pinto, Juliana Guerra ^[1] ; Vitorio, Gabrielle dos Santos ^[1] ; Ferreira, Isabelle ^[1] ; Pacheco-Soares, Cristina ^[2] ; Ariel Mamone, Leandro ^{[3, 4]} ; Strixino, Juliana Ferreira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Photobiol Appl Hlth, Av Shishima Hifumi 2911, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Lab Cellular Dynam, Av Shishima Hifumi 2911, Sao Jose Dos Campos, SP - Brazil

^[3] Consejo Nacl Invest Cient & Tecn, Ctr Invest Porfirinas & Porfirias CIPYP, Cordoba 2351 1er Subsuelo, CP1120AAF, Buenos Aires, DF - Argentina

^[4] Hosp Clin Jose San Martin, Cordoba 2351 1er Subsuelo, CP1120AAF, Buenos Aires, DF - Argentina

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 37, MAR 2022.
• Citações Web of Science: 0

Resumo

Photodynamic Therapy (PDT) is an oncologic treatment, producing reactive oxygen species (ROS) to induce the death of cancer cells. This study aimed to evaluate the action of PDT on gliosarcoma cells, using protoporphyrin IX as PS by incubation with the precursor aminolevulinic acid (ALA). An LED device was used with a light dose of 10 J/cm2. The success of the therapy proved to be dependent on the concentration of ALA, and an incubation time of 4 h required for an effective response. Cell death was prevalent due to necrosis when assessed 18 h postPDT. ALA proved to be an option to PDT in cells of the 9 L/lacZ, with the protocol tested. (AU)

• Processo FAPESP: 16/20022-7 - Comparação dos efeitos da clorina e ALA aplicado à Terapia Fotodinâmica em Gliossarcoma.
• Beneficiário: Leticia Corrêa Fontana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs