Photodynamic effect of protoporphyrin IX in gliosarcoma 9l/lacZ cell line

Author(s)

Fontana, Leticia Correa ^[1] ; Pinto, Juliana Guerra ^[1] ; Vitorio, Gabrielle dos Santos ^[1] ; Ferreira, Isabelle ^[1] ; Pacheco-Soares, Cristina ^[2] ; Ariel Mamone, Leandro ^{[3, 4]} ; Strixino, Juliana Ferreira ^[1]

Affiliation

^[1] Univ Vale Paraiba, Photobiol Appl Hlth, Av Shishima Hifumi 2911, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Lab Cellular Dynam, Av Shishima Hifumi 2911, Sao Jose Dos Campos, SP - Brazil

^[3] Consejo Nacl Invest Cient & Tecn, Ctr Invest Porfirinas & Porfirias CIPYP, Cordoba 2351 1er Subsuelo, CP1120AAF, Buenos Aires, DF - Argentina

^[4] Hosp Clin Jose San Martin, Cordoba 2351 1er Subsuelo, CP1120AAF, Buenos Aires, DF - Argentina

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 37, MAR 2022.
• Web of Science Citations: 0

Abstract

Photodynamic Therapy (PDT) is an oncologic treatment, producing reactive oxygen species (ROS) to induce the death of cancer cells. This study aimed to evaluate the action of PDT on gliosarcoma cells, using protoporphyrin IX as PS by incubation with the precursor aminolevulinic acid (ALA). An LED device was used with a light dose of 10 J/cm2. The success of the therapy proved to be dependent on the concentration of ALA, and an incubation time of 4 h required for an effective response. Cell death was prevalent due to necrosis when assessed 18 h postPDT. ALA proved to be an option to PDT in cells of the 9 L/lacZ, with the protocol tested. (AU)

• FAPESP's process: 16/20022-7 - Comparison of the chlorin Therapy effect with Photodithazine and ALA applied in Gliosarcoma.
• Grantee: Leticia Corrêa Fontana
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC