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Comparison of the chlorin therapy effect with Photodithazine and ALA applied in gliosarcoma

Grant number: 16/20022-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): February 01, 2017
Effective date (End): January 31, 2020
Field of knowledge:Biological Sciences - Biophysics
Principal Investigator:Juliana Ferreira Strixino
Grantee:Leticia Corrêa Fontana
Home Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

Even with the advances in conventional treatment techniques, the prognosis of cancer's in the nervous system follows not conducive to patient, what makes necessary studies of alternative therapies that can be used as primary treatment or concurrent to existing methods. The Photodynamic Therapy (PDT) presents itself as a promising therapy, employing a photosensitive agent (FS), an source of light at an appropriate wavelength to the FS and molecular oxygen, producing reactive oxygen species for the purpose of inducing cancer cell death by necrosis or apoptosis. The objective of this study is the evaluation of the effects of PDT in gliosarcoma cell's using as FS chlorine Photodithazine® (PDZ) and the precursor of protoporphyrin IX, aminolevulinic acid (ALA). The project will be divided as one part in vitro and another in vivo. For the in vitro analysis, the source of light used will be a LED device (Biopdi/Irrad-Led 660) for the PDZ and a LED device (Biopdi/Irrad-Led 630) for the ALA, both formed of 54 LEDs with 70 mW of power in the region of 660 nm and 630 nm, respectively. As for the analysis in vivo will be used a diode laser (QuantumTech®), with intensity of 100 mW / cm and 100 Jcm, at a wavelength of 670 nm for the PDZ and 630 nm for the ALA. Cell viability tests will be conducted, debugging time of the FS, Analysis of the types of the induced cell death, debugging time of the FS in healthy cells, morphological analysis and FS location in confocal microscope. For the in vivo analysis will be held histology of animals with tumor xenograft model, to compare the action of the FS's PDZ and ALA on the tissue and monitoring an experimental group performing the surgical removal of the tumor, followed by the application of PDT, and having the animals followed for 30 days to observe the recovery of the animal, followed by histological analysis.