Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution

Autor(es)

Perez, Caroline Lopes ^{[1, 2]} ; Casciatori, Fernanda Perpetua ^[2] ; Thomeo, Joao Claudio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Food Engn & Technol Dept, Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Grad Program Chem Engn, Rod Washington Luiz Km 235, SP-310, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 44, n. 3, p. 537-548, MAR 2021.
• Citações Web of Science: 1

Resumo

Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses. (AU)

• Processo FAPESP: 14/23453-3 - Ampliação de escala de biorreatores de leito empacotado para a produção de celulases por fermentação em estado sólido
• Beneficiário: João Cláudio Thoméo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 18/00996-2 - Desenvolvimento de um biorreator de leito empacotado pseudo-contínuo para fermentação em estado sólido
• Beneficiário: Fernanda Perpétua Casciatori
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular