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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution

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Author(s):
Perez, Caroline Lopes [1, 2] ; Casciatori, Fernanda Perpetua [2] ; Thomeo, Joao Claudio [1]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Food Engn & Technol Dept, Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Grad Program Chem Engn, Rod Washington Luiz Km 235, SP-310, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Bioprocess and Biosystems Engineering; v. 44, n. 3, p. 537-548, MAR 2021.
Web of Science Citations: 1
Abstract

Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses. (AU)

FAPESP's process: 14/23453-3 - Packed-bed solid-state fermentation bioreactor scale-up for cellulase production
Grantee:João Cláudio Thoméo
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 18/00996-2 - Development of a pseudo-continuous packed-bed bioreactor for solid-state fermentation
Grantee:Fernanda Perpétua Casciatori
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants