Development of a pseudo-continuous packed-bed bioreactor for solid-state fermentation

• Grant number: 18/00996-2
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: May 01, 2018
• End date: April 30, 2020
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Fernanda Perpétua Casciatori
• Grantee: Fernanda Perpétua Casciatori
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Cristiane Sanchez Farinas

Abstract

Developing renewable energetic matrices became strategic at a current scenario in which environmental sustainability appeared as a great global concern. In this context, in Brazil, the biorefinery emerges as an industry centered in the production of biofuels, bioethanol and biodiesel, in parallel with the production of other biomolecules with high added-value. Focusing the biofuels synthesis by the biochemical route, the in-house production of useful enzymes for obtaining bioethanol and biodiesel may assure self-supply. For that, solid-state fermentation (SSF) shows as feasible alternative for these enzymes production as well as for production of other molecules interesting to biorefineries as inputs or final products, employing as raw materials sub or co-products of the biorefinery itself. On the above, it is proposed this regular research project focused on building and operation of a packed-bed bioreactor for pseudo-continuous production of enzymes and other biomolecules by SSF. It will contemplate experimental tests and modeling and simulation in a modular packed-bed bioreactor here named pseudo-continuous, that will be operated as a plug flow reactor. In this reactor, modules will be moved ascendingly like clusters that will compose the body of the packed-bed. After an initial transient period, once the desired bed height is achieved, steady-state operation will be reached. The instrumentation of the equipment aiming monitoring and control is also contemplated. At the end of the project, it is expected to have a SSF bioreactor feasible, patentable and industrially applicable, once it is novel in experimental aspect. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, 2020. (13/01756-1, 18/00996-2, 17/16482-5, 11/07453-5, 10/12624-0, 11/21239-6)

RODRIGUES, NATALIA A.; KATAYAMA, ERIC T.; CASCIATORI, FERNANDA P.. Alternative strategies to perform solid-state cultivation in a multilayer packed-bed bioreactor: Continuous and cyclic operations. CHEMICAL ENGINEERING JOURNAL, v. 448, p. 12-pg., 2022-11-15. (18/16689-1, 18/26097-4, 18/00996-2, 16/10636-8)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, p. 19-pg., 2020-01-01. (17/16482-5, 11/07453-5, 13/01756-1, 10/12624-0, 18/00996-2, 11/21239-6)

DA SILVA, MIRELLA P. DINIZ; DUTRA, RODRIGO SILVA; CASCIATORI, FERNANDA PERPETUA; GRAJALES, LINA MARIA. A two-phase model for simulation of water transfer during lipase production by solid-state cultivation in a tray bioreactor using babassu residues as substrate. CHEMICAL ENGINEERING AND PROCESSING, v. 177, p. 14-pg., 2022-07-01. (16/10636-8, 18/26097-4, 18/00996-2, 18/16689-1)

KATAYAMA, ERIC; RODRIGUES, NATALIA A.; BILATTO, STANLEY; CASCIATORI, FERNANDA P.; FARINAS, CRISTIANE S.. Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. BIOMASS CONVERSION AND BIOREFINERY, v. N/A, p. 16-pg., 2022-06-30. (16/10636-8, 18/00996-2, 18/10899-4, 19/25261-8)

PEREZ, CAROLINE LOPES; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO. Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution. Bioprocess and Biosystems Engineering, v. 44, n. 3, p. 537-548, MAR 2021. (14/23453-3, 18/00996-2)

DOS SANTOS GOMES, A. C.; CASCIATORI, F. P.; GOMES, E.; DA COSTA CARREIRA NUNES, C.; MORETTI, M. M. S.; THOMEO, J. C.. Growth kinetics ofMyceliophthora thermophilaM.7 center dot 7 in solid-state cultivation. Journal of Applied Microbiology, v. 130, n. 1, JUL 2020. (14/23453-3, 18/00996-2, 10/12624-0)

FAVARO, CAMILA P.; BARALDI, ILTON J.; CASCIATORI, FERNANDA P.; FARINAS, CRISTIANE S.. beta-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing. BIOMOLECULES, v. 10, n. 2, FEB 2020. (16/10636-8, 18/00996-2)

CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO. Heat transfer in packed-beds of agricultural waste with low rates of air flow applicable to solid-state fermentation. Chemical Engineering Science, v. 188, p. 97-111, OCT 12 2018. (14/25183-3, 12/13939-0, 14/23453-3, 18/00996-2, 11/07453-5)

DA CUNHA, LUCAS PORTILHO; CASCIATORI, FERNANDA PERPETUA; LOPES, ISABELLA DE CENCO; THOMEO, JOAO CLAUDIO. Production of conidia of the entomopathogenic fungus Metarhizium anisopliae ICB 425 in a tray bioreactor. Bioprocess and Biosystems Engineering, v. 42, n. 11, p. 1757-1768, NOV 2019. (18/00996-2)

DA CUNHA, LUCAS PORTILHO; CASCIATORI, FERNANDA PERPETUA; VICENTE, ISABELA VALENTE; GARCIA, RENATA LAURITO; THOMEO, JOAO CLAUDIO. Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations. Process Biochemistry, v. 97, p. 8-pg., 2020-10-01. (10/12624-0, 18/00996-2, 14/23453-3)