rotein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones

Autor(es)

Silva, Norton Felipe dos Santos ^{[1, 2]} ; Willemart, Rodrigo Hirata ^{[3, 4]} ; da Silva, Jose Roberto Machado Cunha ^{[1, 2]} ; da Silva Junior, Pedro Ismael ^{[2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed ICB 1, Lab Histofisiol Evolut LHE, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed ICB 1, Programa Posgrad Biol Sistemas, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Lab Ecol Sensorial & Comportamento Artropodes LES, BR-03828000 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biociencias, Programa Posgrad Zool, BR-05508090 Sao Paulo, SP - Brazil

^[5] Inst Butantan, Lab Toxinol Aplicada LETA, BR-05503900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS OMEGA; DEC 2021.
• Citações Web of Science: 0

Resumo

The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter-coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of similar to 28, 62, and similar to 198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature. We were able to identify 147 proteins in the inner region, 91 proteins from the outer dorsal region, and 36 proteins from the outer ventral region. Some proteins are present only in one of these regions and some are shared by one or more regions. Our work provides, to the best of our knowledge, the first proteome characterization of the content of an arthrodial membrane gland in arachnids. Dataset Identifier: ftp://massive.ucsd.edu/MSV000087195/. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 20/05158-5 - Defesas morfológicas, comportamentais e químicas contra inimigos naturais em Opiliões (Arachnida)
• Beneficiário: Rodrigo Hirata Willemart
• Modalidade de apoio: Auxílio à Pesquisa - Regular