Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique

Autor(es)

Citta, M. ^[1] ; Anovazzi, G. ^[2] ; Basso, F. G. ^[1] ; Scheffel, D. L. S. ^[3] ; Zhou, J. ^[4] ; Pashley, D. H. ^[5] ; Souza Costa, C. A. ^[6] ; Hebling, J. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Oral Rehabil, Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo, SP - Brazil

^[3] Univ Estadual Maringa, Sch Dent, Dept Dent, Maringa, Parana - Brazil

^[4] Peking Univ, Dept Prosthodont, Sch & Hosp Stomatol, Beijing - Peoples R China

^[5] Augusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA - USA

^[6] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Operative Dentistry; v. 46, n. 6 NOV-DEC 2021.
• Citações Web of Science: 0

Resumo

Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging. (AU)

• Processo FAPESP: 17/03927-9 - Estabilidade mecânica e atividade proteolítica da união resina-dentina produzida pela técnica de adesão seca à dentina biomodificada por cross-linkers
• Beneficiário: Mariana Citta
• Modalidade de apoio: Bolsas no Brasil - Mestrado