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Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility

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Autor(es):
Campos Arcuri, Mariana de Lara [1] ; Nunes-Laitz, Alessandra Vasconcellos [2, 1] ; Macedo Lima, Romulo Pedro [1] ; Barreto, Pedro [3] ; Marinho, Andressa Nagatani [1] ; Arruda, Paulo [3] ; Maia, Ivan G. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP, Dept Ciencias Quim & Biol, Inst Biociencias Botucatu, R Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil
[2] Inst Fed Educ Ciencia & Tecnol Rondonia, Campus Colorado do Oeste, Colorado Do Oeste - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Av Candid Rondon 400, BR-13083875 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Plant and Cell Physiology; v. 62, n. 10, p. 1630-1644, OCT 2021.
Citações Web of Science: 0
Resumo

Mitochondrial uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that dissipate the proton electrochemical gradient generated by the respiratory chain complexes. In plants, these proteins are crucial for maintaining mitochondrial reactive oxygen species (ROS) homeostasis. In this study, single T-DNA insertion mutants for two (AtUCP1 and AtUCP2) out of the three UCP genes present in Arabidopsis thaliana were employed to elucidate their potential roles in planta. Our data revealed a significant increase in the Adenosine triphosphate (ATP)/Adenosine diphosphate (ADP) ratios of both mutants, indicating clear alterations in energy metabolism, and a reduced respiratory rate in atucp2. Phenotypic characterization revealed that atucp1 and atucp2 plants displayed reduced primary root growth under normal and stressed conditions. Moreover, a reduced fertility phenotype was observed in both mutants, which exhibited an increased number of sterile siliques and a lower seed yield compared with wild-type plants. Reciprocal crosses demonstrated that both male fertility and female fertility were compromised in atucp1, while such effect was exclusively observed in the male counterpart in atucp2. Most strikingly, a pronounced accumulation of hydrogen peroxide in the reproductive organs was observed in all mutant lines, indicating a disturbance in ROS homeostasis of mutant flowers. Accordingly, the atucp1 and atucp2 mutants exhibited higher levels of ROS in pollen grains. Further, alternative oxidase 1a was highly induced in mutant flowers, while the expression profiles of transcription factors implicated in gene regulation during female and male reproductive organ/tissue development were perturbed. Overall, these data support the important role for AtUCP1 and AtUCP2 in flower oxidative homeostasis and overall plant fertility. (AU)

Processo FAPESP: 17/25139-2 - Os transportadores de dicarboxilato (DICs) e as proteínas mitocondriais desacopladoras (UCPs) como alvos para o aumento da tolerância das plantas aos estresses abióticos
Beneficiário:Ivan de Godoy Maia
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/19021-1 - Avaliação funcional das proteínas desacopladoras mitocondriais em Arabidopsis thaliana utilizando mutantes de inserção
Beneficiário:Rômulo Pedro Macêdo Lima
Modalidade de apoio: Bolsas no Brasil - Doutorado