| Full text | |
| Author(s): |
Campos Arcuri, Mariana de Lara
[1]
;
Nunes-Laitz, Alessandra Vasconcellos
[2, 1]
;
Macedo Lima, Romulo Pedro
[1]
;
Barreto, Pedro
[3]
;
Marinho, Andressa Nagatani
[1]
;
Arruda, Paulo
[3]
;
Maia, Ivan G.
[1]
Total Authors: 7
|
| Affiliation: | [1] UNESP, Dept Ciencias Quim & Biol, Inst Biociencias Botucatu, R Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil
[2] Inst Fed Educ Ciencia & Tecnol Rondonia, Campus Colorado do Oeste, Colorado Do Oeste - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Av Candid Rondon 400, BR-13083875 Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Plant and Cell Physiology; v. 62, n. 10, p. 1630-1644, OCT 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Mitochondrial uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that dissipate the proton electrochemical gradient generated by the respiratory chain complexes. In plants, these proteins are crucial for maintaining mitochondrial reactive oxygen species (ROS) homeostasis. In this study, single T-DNA insertion mutants for two (AtUCP1 and AtUCP2) out of the three UCP genes present in Arabidopsis thaliana were employed to elucidate their potential roles in planta. Our data revealed a significant increase in the Adenosine triphosphate (ATP)/Adenosine diphosphate (ADP) ratios of both mutants, indicating clear alterations in energy metabolism, and a reduced respiratory rate in atucp2. Phenotypic characterization revealed that atucp1 and atucp2 plants displayed reduced primary root growth under normal and stressed conditions. Moreover, a reduced fertility phenotype was observed in both mutants, which exhibited an increased number of sterile siliques and a lower seed yield compared with wild-type plants. Reciprocal crosses demonstrated that both male fertility and female fertility were compromised in atucp1, while such effect was exclusively observed in the male counterpart in atucp2. Most strikingly, a pronounced accumulation of hydrogen peroxide in the reproductive organs was observed in all mutant lines, indicating a disturbance in ROS homeostasis of mutant flowers. Accordingly, the atucp1 and atucp2 mutants exhibited higher levels of ROS in pollen grains. Further, alternative oxidase 1a was highly induced in mutant flowers, while the expression profiles of transcription factors implicated in gene regulation during female and male reproductive organ/tissue development were perturbed. Overall, these data support the important role for AtUCP1 and AtUCP2 in flower oxidative homeostasis and overall plant fertility. (AU) | |
| FAPESP's process: | 17/25139-2 - Mitochondrial dicarboxylate carriers and mitochondrial uncoupling proteins (UCP) as targets for improving plant tolerance to abiotic stress |
| Grantee: | Ivan de Godoy Maia |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/19021-1 - Functional evaluation of the mitochondrial uncoupling proteins from Arabidopsis thaliana using insertion mutants |
| Grantee: | Rômulo Pedro Macêdo Lima |
| Support Opportunities: | Scholarships in Brazil - Doctorate |