Serum Metabolic Profiles Based on Nuclear Magnetic Resonance Spectroscopy among Patients with Deep Vein Thrombosis and Healthy Controls

Autor(es)

Escobar, Melissa Quintero ^{[1, 2]} ; Tasic, Ljubica ^[2] ; Costa, Tassia Brena Barroso Carneiro da ^[2] ; Stanisic, Danijela ^[2] ; Montalvao, Silmara ^[1] ; Huber, Stephany ^[1] ; Annichino-Bizzacchi, Joyce Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, Hemoctr, BR-13083878 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Chem, Dept Organ Chem, Chem Biol Lab, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 11, n. 12 DEC 2021.
• Citações Web of Science: 0

Resumo

Deep venous thrombosis (DVT) is associated with significant morbidity and mortality. Studies on changes in the level of metabolites could have the potential to reveal biomarkers that can assist in the early detection, diagnosis, monitoring of DVT progression, response to treatment, or recurrence of DVT. In this scenario, the metabolomic analysis can provide a better understanding of the biochemical dysregulations of thrombosis. Using an untargeted metabolomic approach through magnetic resonance spectroscopy and multi- and univariate statistical analysis, we compared 40 patients with previous venous thrombosis and 40 healthy individuals, and we showed important serum differences between patients and controls, especially in the spectral regions that correspond to glucose, lipids, unsaturated lipids, and glycoprotein A. Considering the groups depending on risk factors and the local of the previous episode (lower limbs or cerebral system), we also noticed differences in metabolites linked to lipids and lactate. Comparative analyses pointed to altered ratios of glucose/lactate and branched-chain amino acids (BCAAs)/alanine, which might be associated with the fingerprints of thrombosis. Although samples for metabolomic analysis were collected months after the acute episode, these results highlighted that, alterations can still remain and may contribute to a better understanding of the complications of the disease. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/14172-6 - Investigação de aspectos fisiopatológicos e novas abordagens terapêuticas em doenças tromboembólicas
• Beneficiário: Joyce Maria Annichino-Bizzacchi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/18624-4 - Análises metabolômicas e metabonômicas por técnicas de ressonância magnética nuclear aplicadas em estudos da trombose venosa
• Beneficiário: Melissa Quintero Escobar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado