Serum Metabolic Profiles Based on Nuclear Magnetic Resonance Spectroscopy among Patients with Deep Vein Thrombosis and Healthy Controls

Author(s)

Escobar, Melissa Quintero ^{[1, 2]} ; Tasic, Ljubica ^[2] ; Costa, Tassia Brena Barroso Carneiro da ^[2] ; Stanisic, Danijela ^[2] ; Montalvao, Silmara ^[1] ; Huber, Stephany ^[1] ; Annichino-Bizzacchi, Joyce Maria ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, Hemoctr, BR-13083878 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Chem, Dept Organ Chem, Chem Biol Lab, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: METABOLITES; v. 11, n. 12 DEC 2021.
• Web of Science Citations: 0

Abstract

Deep venous thrombosis (DVT) is associated with significant morbidity and mortality. Studies on changes in the level of metabolites could have the potential to reveal biomarkers that can assist in the early detection, diagnosis, monitoring of DVT progression, response to treatment, or recurrence of DVT. In this scenario, the metabolomic analysis can provide a better understanding of the biochemical dysregulations of thrombosis. Using an untargeted metabolomic approach through magnetic resonance spectroscopy and multi- and univariate statistical analysis, we compared 40 patients with previous venous thrombosis and 40 healthy individuals, and we showed important serum differences between patients and controls, especially in the spectral regions that correspond to glucose, lipids, unsaturated lipids, and glycoprotein A. Considering the groups depending on risk factors and the local of the previous episode (lower limbs or cerebral system), we also noticed differences in metabolites linked to lipids and lactate. Comparative analyses pointed to altered ratios of glucose/lactate and branched-chain amino acids (BCAAs)/alanine, which might be associated with the fingerprints of thrombosis. Although samples for metabolomic analysis were collected months after the acute episode, these results highlighted that, alterations can still remain and may contribute to a better understanding of the complications of the disease. (AU)

• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/14172-6 - Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders
• Grantee: Joyce Maria Annichino-Bizzacchi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/18624-4 - Metabolomic and metabonomic analyzes by nuclear magnetic resonance techniques applied in studies of venous thrombosis
• Grantee: Melissa Quintero Escobar
• Support Opportunities: Scholarships in Brazil - Post-Doctoral