Campylobacter coli strains from Brazil can invade phagocytic and epithelial cells and induce IL-8 secretion

Autor(es)

Gomes, Carolina N. ^[1] ; Campioni, Fabio ^[1] ; Vilela, Felipe P. ^[1] ; Duque, Sheila S. ^[2] ; Falcao, Juliana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, Campus Univ USP, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Inst Oswaldo Cruz IOC, Fundacao Oswaldo Cruz FIOCRUZ, Pavilhao Rocha Lima, Sala 516, Ave Brasil 4365, BR-21040900 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 52, n. 2, p. 859-867, JUN 2021.
• Citações Web of Science: 0

Resumo

Campylobacter spp. have been a predominant cause of bacterial foodborne gastroenteritis worldwide, causing substantial costs to public healthcare systems. This study aimed to assess the invasion and pro-inflammatory cytokine production capacity of Campylobacter coli strains isolated in Brazil. A total of 50 C. coli isolated from different sources in Brazil were analyzed for their capacity of invasion in Caco-2 and U-937 cell lines. The production of pro-inflammatory cytokines was quantitatively measured in response to C. coli. All the strains studied showed invasion percentage >= 40% in polarized Caco-2 cells. In U-937 cells assay, 35 of 50 C. coli strains studied showed invasion percentage >= 50%. A significant increase in IL-8 production by infected U-937 cells was observed for 17.5% of the C. coli isolates. The high percentages of invasion in Caco-2 and U-937 cells observed for all studied strains, plus the increased production of IL-8 by U-937 cells against some strains, highlighted the pathogenic potential of the C. coli studied and bring extremely relevant data since it has never been reported for strains isolated in Brazil and there are a few data for C. coli in the literature. (AU)

• Processo FAPESP: 15/23408-0 - Análise fenotípica, sequenciamento do genoma e transcriptoma de linhagens de Campylobacter coli isoladas de diversas fontes durante 16 anos no Brasil
• Beneficiário: Carolina Nogueira Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/24716-3 - Sequenciamento do genoma, transcriptoma e análise fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular