| Texto completo | |
| Autor(es): |
Agalya, P.
[1]
;
de Oliveira, I. Pires
[2, 3]
;
Lescano, C. H.
[4]
;
Caires, A. R. L.
[5]
;
Velusamy, V.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Arignar Anna Govt Arts Coll, PG & Res Dept Phys, Namakkal 637002, TN - India
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[3] Univ Fed Minas Gerais, Inst Agr Sci, BR-39400149 Montes Claros, MG - Brazil
[4] Univ Estadual Campinas, Sch Med Sci, Dept Pharmacol, Campinas, SP - Brazil
[5] Fed Univ MatoGrosso do Sul, Inst Phys, CP 549, BR-79070900 Campo Grande, MS - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FOOD HYDROCOLLOIDS; v. 125, APR 2022. |
| Citações Web of Science: | 0 |
| Resumo | |
The protein-sucralose (SL) interaction is getting more attention in food and pharmaceutical products due to the peculiar properties of SL compared to the normal sugars. This article discusses the effect of SL as an additive combined with pH variation on the solvation profile of Ovalbumin (OVA) by molecular interaction studies. The findings were explored experimentally by measuring density, ultrasound velocity, viscosity, surface tension and further extended from the analysis of some thermo-acoustical parameters. Results suggested that cosolvent SL interact with OVA in larger magnitude in acidic pH region whereas lesser in alkaline pH region. In addition, results confirm the presence of dipole-dipole interactions exists in both pH regions but differ in their magnitude. The simulation results suggest that the solvent (water)/cosolvent (SL) accumulation on the OVA surface has a pH-dependence behavior. The MD coupled to minimum distance distribution function g(md) (r) showed an accumulation of SL closer to the OVA surface at similar to 1.8 angstrom and water at similar to 2.5 angstrom, suggesting that SL structure plays a vital role in the weakening of the preferential exclusion of itself from the OVA surface. The prominent existence of non-covalent interactions especially hydrogen bonding and van der Waals interactions are confirmed by both experimental and computational studies thereby leads to OVA denaturation in both acidic and alkaline regions to a different extent. (AU) | |
| Processo FAPESP: | 17/26687-3 - Caracterização farmacológica das gliflozinas em plaquetas humanas isoladas: avaliação in vitro, in vivo e modelagem molecular |
| Beneficiário: | Caroline Honaiser Lescano |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/02201-4 - Estudo da inibição do acúmulo de nucleotídeos cíclicos por derivados de piridopirimidinas usando simulações de dinâmica molecular |
| Beneficiário: | Ivan Pires de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |