ype 1 Innate Lymphoid Cell and Natural Killer Cells Are Sources of Interferon-gamma and Other Inflammatory Cytokines Associated With Distinct Clinical Presentation in Early Dengue Infectio

Autor(es)

Quintino-de-Carvalho, Iracema Luisa ^[1] ; Goncalves-Pereira, Marcela Helena ^[2] ; Ramos, Michele Faria ^[2] ; Goncalves de Aguiar Milhim, Bruno Henrique ^[3] ; Da Costa, Ultimo Libanio ^[4] ; Santos, Erika Goncalves ^[4] ; Nogueira, Mauricio Lacerda ^[3] ; Santiago, Helton Da Costa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG - Brazil

^[2] Univ Fed Minas Gerais, Dept Imunol & Bioquim, Belo Horizonte, MG - Brazil

^[3] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[4] Secretaria Municipal Saude, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Infectious Diseases; v. 225, n. 1, p. 84-93, JAN 1 2022.
• Citações Web of Science: 0

Resumo

Background. Increased levels of inflammatory cytokines are associated with severe dengue evolution, but the source of such hypercytokinemia is elusive. We investigated the contribution of innate lymphocytes, innate lymphoid cells (ILCs), and natural killer (NK) cells in cytokine production in early dengue infection. Methods. Peripheral blood mononuclear cells of individuals with dengue without warning signs (DWS-) and dengue with warning signs and severe dengue (SD) presentation combined (DWS+) were obtained between 2 and 7 days since fever onset and submitted to flow cytometry without specific antigen stimulation to evaluate cytokines in ILC and NK cell subpopulations. Results. ILCs and NK cells were found to be important sources of cytokines during dengue. ILCs of the DWS+/SD group displayed higher production of interferon gamma (IFN-gamma) and interleukin (IL) 4/IL-13 when compared to DWS- individuals. On the other hand, NK Eomes(+) cells of DWS- patients displayed higher IFN-gamma production levels compared with the DWS+/SD group. Interestingly, when NK cells were identified by CD56 expression, DWS+/SD displayed higher frequency of IL-17 production compared with the DWS- group. Conclusions. Mese results indicate that ILCs and NK cells are important sources of inflammatory cytokines during acute dengue infection and display distinct profiles associated with different clinical forms. (AU)

• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático