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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ype 1 Innate Lymphoid Cell and Natural Killer Cells Are Sources of Interferon-gamma and Other Inflammatory Cytokines Associated With Distinct Clinical Presentation in Early Dengue Infectio

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Author(s):
Quintino-de-Carvalho, Iracema Luisa [1] ; Goncalves-Pereira, Marcela Helena [2] ; Ramos, Michele Faria [2] ; Goncalves de Aguiar Milhim, Bruno Henrique [3] ; Da Costa, Ultimo Libanio [4] ; Santos, Erika Goncalves [4] ; Nogueira, Mauricio Lacerda [3] ; Santiago, Helton Da Costa [2]
Total Authors: 8
Affiliation:
[1] Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG - Brazil
[2] Univ Fed Minas Gerais, Dept Imunol & Bioquim, Belo Horizonte, MG - Brazil
[3] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil
[4] Secretaria Municipal Saude, Belo Horizonte, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Infectious Diseases; v. 225, n. 1, p. 84-93, JAN 1 2022.
Web of Science Citations: 0
Abstract

Background. Increased levels of inflammatory cytokines are associated with severe dengue evolution, but the source of such hypercytokinemia is elusive. We investigated the contribution of innate lymphocytes, innate lymphoid cells (ILCs), and natural killer (NK) cells in cytokine production in early dengue infection. Methods. Peripheral blood mononuclear cells of individuals with dengue without warning signs (DWS-) and dengue with warning signs and severe dengue (SD) presentation combined (DWS+) were obtained between 2 and 7 days since fever onset and submitted to flow cytometry without specific antigen stimulation to evaluate cytokines in ILC and NK cell subpopulations. Results. ILCs and NK cells were found to be important sources of cytokines during dengue. ILCs of the DWS+/SD group displayed higher production of interferon gamma (IFN-gamma) and interleukin (IL) 4/IL-13 when compared to DWS- individuals. On the other hand, NK Eomes(+) cells of DWS- patients displayed higher IFN-gamma production levels compared with the DWS+/SD group. Interestingly, when NK cells were identified by CD56 expression, DWS+/SD displayed higher frequency of IL-17 production compared with the DWS- group. Conclusions. Mese results indicate that ILCs and NK cells are important sources of inflammatory cytokines during acute dengue infection and display distinct profiles associated with different clinical forms. (AU)

FAPESP's process: 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018
Grantee:Maurício Lacerda Nogueira
Support Opportunities: Research Projects - Thematic Grants