ytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cell

Autor(es)

Pedrosa, Marlus da Silva ^[1] ; Alves, Tomaz ^[2] ; Rahhal, Juliana Garuba ^[3] ; Nogueira, Fernando Neves ^[1] ; Sipert, Carla Renata ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PESQUISA BRASILEIRA EM ODONTOPEDIATRIA E CLINICA INTEGRADA; v. 22, 2022.
• Citações Web of Science: 0

Resumo

Objective: To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods: The culture of hPDLSCs was established. Cell density was set at 2 x 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (alpha=0.05). Results: At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion: Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs. (AU)

• Processo FAPESP: 17/23158-0 - Efeito da ativação do receptor ativado por protease do tipo 1 (par1) sobre a atividade osteogênica em membranas de células-tronco do ligamento periodontal humano
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro
• Beneficiário: Carla Renata Sipert
• Modalidade de apoio: Auxílio à Pesquisa - Regular