olishing entire stems and roots using sandpaper under water: An alternative method for macroscopic analyse

Autor(es)

Barbosa, Antonio C. F. ^[1] ; Gerolamo, Caian S. ^[2] ; Lima, Andre C. ^[3] ; Angyalossy, Veronica ^[2] ; Pace, Marcelo R. ^[4]

Afiliação do(s) autor(es)

^[1] Inst Pesquisas Tecnol, Ctr Tecnol Recursos Florestais, Lab Madeira & Prod Derivados, Av Prof Almeida Prado 532, Cidade Univ, BR-05508901 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Anat Vegetal, Inst Biociencias, Dept Bot, Rua Matao 277, Cidade Univ, BR-05508090 Sao Paulo - Brazil

^[3] Weizmann Inst Sci, Dept Plant & Environm Sci, Weizmann Tree Lab, Herzl St 234, IL-76100 Rehovot - Israel

^[4] Univ Nacl Autonoma Mexico, Inst Biol, Dept Bot, Circuito Zona Deport S-N Ciudad Univ, Mexico City 04510, DF - Mexico

• Tipo de documento: Artigo Científico
• Fonte: APPLICATIONS IN PLANT SCIENCES; v. 9, n. 5 MAY 2021.
• Citações Web of Science: 1

Resumo

PREMISE: Polishing entire stem and root samples is an effective method for studying their anatomy; however, polishing fresh samples to preserve woods with soft tissues or barks is challenging given that soft tissues shrink when dried. We propose sanding fresh or liquid-preserved samples under water as an alternative, given that it preserves all tissues in an intact and clear state. METHODS AND RESULTS: By manually grinding the surface of the samples under water using three ascending grits of waterproof sandpapers, an excellent polished sanded surface is obtained. The wood swarf goes into the water without clogging the cell lumina, rendering the surfaces adequate for cell visualization and description. We show results in palms, liana stems, roots, and wood blocks. CONCLUSIONS: Using this simple, inexpensive, rapid technique, it is possible to polish either fresh, dry, or liquid-preserved woody plant samples, preserving the integrity of both the soft and hard tissues and allowing for detailed observations of the stems and roots. (AU)

• Processo FAPESP: 18/06917-7 - LIANAS: abordagem integrativa sob a perspectiva anatômica
• Beneficiário: Veronica Angyalossy
• Modalidade de apoio: Auxílio à Pesquisa - Regular