pical periodontitis promotes insulin resistance and alters adaptive immunity markers in rat

Autor(es):Mostrar menos -

Pereira, Renato Felipe ^[1] ; Lattari Tessarin, Gestter Willian ^{[2, 3]} ; Chiba, Fernando Yamamoto ^[4] ; de Lima Coutinho Mattera, Maria Sara ^[1] ; Pereira, Amanda Gomes ^[5] ; Saori Tsosura, Thais Veronica ^[1] ; Balera Brito, Victor Gustavo ^[1] ; Fujii de Oliveira, Renan Akira ^[4] ; Ervolino, Edilson ^{[3, 6]} ; Penha de Oliveira, Sandra Helena ^[1] ; Angelo Cintra, Luciano Tavares ^[4] ; Matsushita, Doris Hissako ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Programa Posgrad Multicentr Ciencias Fisiol, PPGMCF, UNESP, SBFis, Aracatuba, SP - Brazil

^[2] Univ Ctr North Paulista UNORP, Sao Jose Do Rio Preto, SP - Brazil

^[3] Sao Paulo State Univ, Sch Dent, Dept Basic Sci, UNESP, Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ, Sch Dent, Dept Prevent & Restorat Dent, UNESP, Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ, Botucatu Med Sch, Internal Med Dept, UNESP, Botucatu, SP - Brazil

^[6] Sao Paulo State Univ, Inst Biosci Botucatu, UNESP, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SAUDI DENTAL JOURNAL; v. 33, n. 8, p. 979-986, DEC 2021.
• Citações Web of Science: 0

Resumo

Objective: Apical periodontitis (AP) is a chronic or acute inflammatory disease usually developed from endodontic infections, predominantly due to gram-negative anaerobic bacteria invading the dental pulp. This study aimed to evaluate lymphocyte markers to assess the involvement of adaptive immunity in insulin resistance (IR) in a rat model of AP. Design. Forty-five male Wistar albino rats were divided into 3 groups (control, 1AP and 4AP). AP was induced in the upper right first molar (1AP), and in the first and second upper and lower right molars (4AP). The spleen was collected to evaluate the expression of transcription factors involved in lymphocyte polarization, including T-bet (Th1), GATA3 (Th2), and FOXP3 (Treg). Blood samples were assessed for serum cytokine levels transcribed by the respective lymphocyte polarizations, INF-gamma (Th1), IL-4 (Th2) and TGF-beta (Treg). In addition, glucose and insulin levels were measured to evaluate IR by the HOMA-IR method. Results: The results showed higher T-bet expression on AP groups, along with lower GATA3 and FOXP3 expression in the 1AP, in addition to increased GATA3 and decreased FOXP3 expression in the 4AP group compared to the CN group. There was no difference in the INF-gamma levels, while IL-4 was decreased in the AP groups. Taken together, these results suggest that the adaptive immune system, with a predominance of the Th1 polarization, may be involved in the development of IR in rats with AP. Conclusions: AP promotes increase in the expression of T-bet (4AP) and decrease of FOXP3 expressions and IL-4 levels (1AP and 4AP). However, depending on the number of lesions (1 or 4 lesions), the expression of GATA3 appears differently. Thus, innate immunity and adaptive immunity may contribute to the IR observed in rats with AP. (C) 2021 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. (AU)

• Processo FAPESP: 16/24829-2 - Avaliação da infiltração de macrófagos, do sinal inflamatório e atrofia em músculo esquelético de ratos com lesão periapical
• Beneficiário: Doris Hissako Matsushita
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/17619-6 - Estudo dos mecanismos envolvidos no desenvolvimento de resistência à insulina em ratos com lesão periapical
• Beneficiário: Renato Felipe Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/17795-0 - Avaliação da infiltração de macrófagos, do sinal inflamatório e atrofia em músculo esquelético de ratos com lesão periapical
• Beneficiário: Andressa Caroline Hernandes Magalhães
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 14/26517-2 - Avaliação do conteúdo da proteína transportadora de glicose GLUT4 em músculo esquelético de ratos adultos com lesão periapical
• Beneficiário: Renan Akira Fujii de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica