eukotriene B-4 loaded in microspheres regulate the expression of genes related to odontoblastic differentiation and biomineralization by dental pulp stem cell

Autor(es)

da Silva, Francine Lorencetti ^[1] ; de Campos Chaves Lamarque, Giuliana ^[2] ; de Oliveira, Fernanda Maria Machado Pereira Cabral ^[2] ; Nelson-Filho, Paulo ^[2] ; da Silva, Lea Assed Bezerra ^[2] ; Segato, Raquel Assed Bezerra ^[2] ; Faccioli, Lucia Helena ^[3] ; Paula-Silva, Francisco Wanderley Garcia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Rio Verde, Rio Verde, Go - Brazil

^[2] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC ORAL HEALTH; v. 22, n. 1 FEB 23 2022.
• Citações Web of Science: 0

Resumo

Background Leukotriene B-4 (LTB4) is a potent lipid mediator that stimulate the immune response. Because dental pulp inflammation and dentin repair are intrinsically related responses, the aim of this research was to investigate the potential of LTB4 in inducing differentiation of dental pulp stem cells. Methods Microspheres (MS) loaded with LTB4 were prepared using an oil emulsion solvent extraction evaporation process and sterility, characterization, efficiency of LTB4 encapsulation and in vitro LTB4 release assay were investigated. Mouse dental pulp stem cells (OD-21) were stimulated with soluble LTB4 or MS loaded with LTB4 (0.01 and 0.1 mu M). Cytotoxicity and cell viability was determined by lactate dehydrogenase and methylthiazol tetrazolium assays. Gene expression were measured by quantitative reverse transcription polymerase chain reaction after 3, 6, 24, 48 and 72 h. Mineralized nodule formation was assessed after 28 days of OD-21 cell stimulation with LTB4 in mineralized media or not. Groups were compared using one-way ANOVA test followed by Dunnett's post-test (alpha = 0.05). Results Treatment with LTB4 or MS loaded with LTB4 (0.01 and 0.1 mu m-mu M) were not cytotoxic to OD-21 cells. Treatment with LTB4 modulated the expression of the Ibsp (integrin binding sialoprotein) and Runx2 (runt-related transcription factor 2) genes differently depending on the experimental period analyzed. Interestingly LTB4 loaded in microspheres (0.1 mu M) allowed long term dental pulp cell differentiation and biomineralization. Conclusion LTB4, soluble or loaded in MS, were not cytotoxic and modulated the expression of the Ibsp and Runx2 genes in cultured OD-21 cells. When LTB4 was incorporated into MS, odontoblast differentiation and mineralization was induced in long term culture. (AU)

• Processo FAPESP: 10/17611-4 - Mecanismos envolvidos na modulação da via 5-lipoxigenase na lesão periapical experimentalmente induzida
• Beneficiário: Francisco Wanderley Garcia de Paula e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores