alleria mellonella for systemic assessment of anti-Candida auris using amphotericin B loaded in nanoemulsio

Autor(es)

Marena, Gabriel Davi ^[1] ; Dos Santos Ramos, Matheus Aparecido ^[1] ; Lima, Laura Caminitti ^[2] ; Chorilli, Marlus ^[1] ; Bauab, Tais Maria ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 807, n. 3 FEB 10 2022.
• Citações Web of Science: 0

Resumo

Galleria mellonella is a model that uses adult larvae to assess the prophylactic, therapeutic, and acute toxic potential of substances. Given their benefits, G. mellonella models are being employed in investigations of systemic infections caused by highly resistant microorganisms. Among the multiresistant microorganisms, we highlight Candida auris, a yeast with high mortality potential and resistance. Among the potential drugs, amphotericin B (AmB) stands out; however, microbial resistance episodes and side effects caused by low selectivity have been observed. The incorporation of AmB into a nanoemulsion (NE) can contribute to the control of C. auris infections and resistance as well as decrease the side effects of this drug. This study aimed to develop AmB-loaded NE (NEA) and evaluate its antifungal action against C. auris in G. mellonella. NEs were obtained by using sunflower oil and cholesterol as the oily phase, polyoxyethylene 20 cetyl ether (Brij (R) 58) and soy phosphatidylcholine as the surfactant system, and PBS buffer as the aqueous phase. An alternative in vivo assay with G. mellonella for acute toxicity and infection was performed using adult stage larvae (200 mg to 400 mg). According to the obtained results, NE and NEA exhibited sizes of 43 and 48 nm, respectively. The PDI was 0.285 and 0.389 for NE and NEA, respectively. The ZP showed electronegativity for both systems, with -3.77 mV and -3.80 mV for NE and NEA, respectively. Acute toxicity showed that free AmB had greater acute toxicity potential than NEA. The survival assay showed high larval viability. NEA had a better antifungal profile against systemic infection in G. mellonella. It is concluded that the alternative model proved to be an efficient in vivo assay to determine the toxicity and evaluate the therapeutic property of free AmB and NEA in systemic infections caused by C. auris. (c) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/23442-2 - Avaliação do potencial de lipossomas para co-encapsulação de anfotericina B e micafungina no tratamento de infecção sistêmica causada por Candida auris
• Beneficiário: Matheus Aparecido dos Santos Ramos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/09831-9 - Nanoemulsões para co-encapsulação de anfotericina B e micafungina: desenvolvimento, caracterização e avaliação do potencial in vitro e in vivo contra Candida auris
• Beneficiário: Gabriel Davi Marena
• Modalidade de apoio: Bolsas no Brasil - Doutorado