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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

alleria mellonella for systemic assessment of anti-Candida auris using amphotericin B loaded in nanoemulsio

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Author(s):
Marena, Gabriel Davi [1] ; Dos Santos Ramos, Matheus Aparecido [1] ; Lima, Laura Caminitti [2] ; Chorilli, Marlus [1] ; Bauab, Tais Maria [2]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Science of The Total Environment; v. 807, n. 3 FEB 10 2022.
Web of Science Citations: 0
Abstract

Galleria mellonella is a model that uses adult larvae to assess the prophylactic, therapeutic, and acute toxic potential of substances. Given their benefits, G. mellonella models are being employed in investigations of systemic infections caused by highly resistant microorganisms. Among the multiresistant microorganisms, we highlight Candida auris, a yeast with high mortality potential and resistance. Among the potential drugs, amphotericin B (AmB) stands out; however, microbial resistance episodes and side effects caused by low selectivity have been observed. The incorporation of AmB into a nanoemulsion (NE) can contribute to the control of C. auris infections and resistance as well as decrease the side effects of this drug. This study aimed to develop AmB-loaded NE (NEA) and evaluate its antifungal action against C. auris in G. mellonella. NEs were obtained by using sunflower oil and cholesterol as the oily phase, polyoxyethylene 20 cetyl ether (Brij (R) 58) and soy phosphatidylcholine as the surfactant system, and PBS buffer as the aqueous phase. An alternative in vivo assay with G. mellonella for acute toxicity and infection was performed using adult stage larvae (200 mg to 400 mg). According to the obtained results, NE and NEA exhibited sizes of 43 and 48 nm, respectively. The PDI was 0.285 and 0.389 for NE and NEA, respectively. The ZP showed electronegativity for both systems, with -3.77 mV and -3.80 mV for NE and NEA, respectively. Acute toxicity showed that free AmB had greater acute toxicity potential than NEA. The survival assay showed high larval viability. NEA had a better antifungal profile against systemic infection in G. mellonella. It is concluded that the alternative model proved to be an efficient in vivo assay to determine the toxicity and evaluate the therapeutic property of free AmB and NEA in systemic infections caused by C. auris. (c) 2021 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 18/23442-2 - Evaluation of the potential of liposomes for co-encapsulation of amphotericin B and micafungin in the treatment of systemic infection caused by Candida auris
Grantee:Matheus Aparecido dos Santos Ramos
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
Grantee:Maria Vitória Lopes Badra Bentley
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/09831-9 - Nanoemulsion for co-encapsulation on amphotericin B and micafungin: development and characterizations of the in vitro and in vivo Candida auris potential.
Grantee:Gabriel Davi Marena
Support Opportunities: Scholarships in Brazil - Doctorate