Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

Autor(es)

Raquel Assed Bezerra Silva ^[1] ; Patrícia Gaton-Hernandez ^[2] ; Carolina Maschietto Pucinelli ^[3] ; Francisco Wanderley Garcia de Paula e Silva ^[4] ; Marília Pacífico Lucisano ^[5] ; Alberto Consolaro ; Rafaela Cardoso de Sá ^[7] ; Lisa Danielly Curcino Araujo ^[8] ; Manoel Damião Sousa-Neto ^[9] ; Léa Assed Bezerra Silva ^[10]

Afiliação do(s) autor(es)

^[1] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[2] University of Barcelona. School of Dentistry. Department of Integrated Paediatric Dentistr - Espanha

^[3] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[4] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[5] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[7] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[8] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[9] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Restorative Dentistry - Brasil

^[10] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 33, n. 1, p. 41-56, 2022-03-07.

Resumo

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice exposed to tricalcium silicate (Biodentine) and aggregated mineral trioxide (ProRoot MTA). A total of 120 mice were divided into 4 groups in 3 different experimental periods (7, 21 and 63 days): Biodentine; Pro-Root MTA; zinc oxide-eugenol and; Negative control - Sham. After the experimental periods microscopic descriptive, semi-quantitative and quantitative analysis of the inflammatory process were analyzed on H&E sections and evaluation of the gene expression of Il10, Infg, Il6, Il1r1 and Tnf (qRT-PCR) were performed. The data obtained were analyzed using the chi-square test and two-way analysis of variance (ANOVA) followed by the Bonferroni post-test (5% significance level). Results: In the microscopic analysis, a slight inflammatory infiltrate was observed, with a predominance of sparse macrophages and polymorphonuclear cells, slight tissue fibrosis, regular fibrous capsule and with dystrophic calcifications, in all groups that received the materials (Biodentine and Pro-Root MTA). In parallel, all materials modulated the gene expression of the different cytokines and receptors evaluated. Conclusion: Pro-Root MTA and Biodentine showed a tissue compatibility, mediated inflammation, with increased fibrous tissue and production of pro- and anti-inflammatory cytokines. (AU)

• Processo FAPESP: 14/25692-5 - Indução da diferenciação de células da polpa dentária e biomineralização: ensaios em cultura celular, em modelos animais e aplicação clínica em humanos
• Beneficiário: Léa Assed Bezerra da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular