Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

Full text
Author(s):
Raquel Assed Bezerra Silva [1] ; Patrícia Gaton-Hernandez [2] ; Carolina Maschietto Pucinelli [3] ; Francisco Wanderley Garcia de Paula e Silva [4] ; Marília Pacífico Lucisano [5] ; Alberto Consolaro ; Rafaela Cardoso de Sá [7] ; Lisa Danielly Curcino Araujo [8] ; Manoel Damião Sousa-Neto [9] ; Léa Assed Bezerra Silva [10]
Total Authors: 10
Affiliation:
[1] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[2] University of Barcelona. School of Dentistry. Department of Integrated Paediatric Dentistr - Espanha
[3] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[4] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[5] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[7] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[8] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
[9] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Restorative Dentistry - Brasil
[10] University of Sao Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil
Total Affiliations: 10
Document type: Journal article
Source: Brazilian Dental Journal; v. 33, n. 1, p. 41-56, 2022-03-07.
Abstract

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice exposed to tricalcium silicate (Biodentine) and aggregated mineral trioxide (ProRoot MTA). A total of 120 mice were divided into 4 groups in 3 different experimental periods (7, 21 and 63 days): Biodentine; Pro-Root MTA; zinc oxide-eugenol and; Negative control - Sham. After the experimental periods microscopic descriptive, semi-quantitative and quantitative analysis of the inflammatory process were analyzed on H&E sections and evaluation of the gene expression of Il10, Infg, Il6, Il1r1 and Tnf (qRT-PCR) were performed. The data obtained were analyzed using the chi-square test and two-way analysis of variance (ANOVA) followed by the Bonferroni post-test (5% significance level). Results: In the microscopic analysis, a slight inflammatory infiltrate was observed, with a predominance of sparse macrophages and polymorphonuclear cells, slight tissue fibrosis, regular fibrous capsule and with dystrophic calcifications, in all groups that received the materials (Biodentine and Pro-Root MTA). In parallel, all materials modulated the gene expression of the different cytokines and receptors evaluated. Conclusion: Pro-Root MTA and Biodentine showed a tissue compatibility, mediated inflammation, with increased fibrous tissue and production of pro- and anti-inflammatory cytokines. (AU)

FAPESP's process: 14/25692-5 - Dental pulp cell differentiation and biomineralization: cell culture assays, experimental models and clinical application in human
Grantee:Léa Assed Bezerra da Silva
Support Opportunities: Regular Research Grants