Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates

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Ana Paula Schappo ^[1] ; Najara C Bittencourt ^[2] ; Leticia P Bertolla ^[3] ; Sofia Forcellini ^[4] ; Ana Beatriz Iung Enembreck da Silva ^[5] ; Hellen Geremias dos Santos ^[6] ; João Henrique Gervásio ^[7] ; Marcus VG Lacerda ; Stefanie CP Lopes ^[9] ; Fabio TM Costa ^[10] ; Letusa Albrecht

Afiliação do(s) autor(es):Mostrar menos -

^[1] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução, Microbiologia e Imunologia - Brasil

^[3] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[4] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[5] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[6] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[7] Fundação Oswaldo Cruz-Fiocruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa - Brasil

^[9] Fundação Oswaldo Cruz-Fiocruz. Instituto Leônidas & Maria Deane - Brasil

^[10] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução, Microbiologia e Imunologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 116, 2022-02-04.

Resumo

BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E. METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells. FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1. MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1. (AU)

• Processo FAPESP: 17/18611-7 - Desenvolvimento de novas ferramentas para busca e validação de alvos moleculares para terapia contra Plasmodium vivax
• Beneficiário: Fabio Trindade Maranhão Costa
• Modalidade de apoio: Auxílio à Pesquisa - Temático