Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture

Autor(es)

Maya Fernanda Manfrin Arnez ^[1] ; Patrícia Maria Monteiro ^[2] ; Francisco Wanderley Garcia Paula-Silva ^[3] ; Gabriel Barretto Dessotti ^[4] ; Luciane Macedo de Menezes ^[5] ; Erika Calvano Küchler ^[6] ; Sandra Yasuyo Fukada Alves ^[7] ; Mirian Aiko Nakane Matsumoto ^[8] ; Maria Bernadete Sasso Stuani ^[9]

Afiliação do(s) autor(es)

^[1] University of Sao Paulo. School of Dentistry of Ribeirao Preto. Department of Othodontic - Brasil

^[2] University of Sao Paulo. School of Dentistry of Ribeirao Preto. Department of Othodontic - Brasil

^[3] University of Sao Paulo. School of Dentistry of Ribeirao Preto. Department of Othodontic - Brasil

^[4] University of Sao Paulo. School of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analysis, Toxicology and Food Science - Brasil

^[5] Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Orthodontics - Brasil

^[6] University Medical Centre of Regensburg. Department of Orthodontics, School of Dentistry - Alemanha

^[7] University of Sao Paulo, Ribeirao Preto. School of Pharmaceutical Sciences of Ribeirão Preto. Department of Physics and Chemistry - Brasil

^[8] University of Sao Paulo. School of Dentistry of Ribeirao Preto. Department of Othodontic - Brasil

^[9] University of Sao Paulo. School of Dentistry of Ribeirao Preto. Department of Othodontic - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 33, n. 2, p. 99-108, 2022-04-29.

Resumo

Abstract Considering that smoking is a public health problem that has been growing among adolescents, the aim of this study was to investigate the impact of cigarette smoke on osteogenic and osteoclastogenic signaling in middle palatal suture of rats. Male Wistar rats exposed (n = 30) or not to cigarette smoke (n = 30) were used. Exposure to smoke was carried out for two daily periods of 3 minutes each, with an interval of 12 hours between exposures. After the experimental periods of 3, 7, 14 and 21 days, the animals were euthanized. The collected tissues were analyzed using light microscopy and real-time RT-PCR was performed to investigate gene expression. The data obtained were compared using the Kruskal Wallis and Dunn tests (⍺ = 5%). Morphologically, there were no significant changes in the middle palatal suture of rats exposed or not to cigarette smoke during 3, 7, 14 and 21 days (p> 0.05). On the other hand, osteoclastogenic signaling was increased in animals exposed to smoke and was characterized by a higher production of RANKL at 3 and 14 days (p <0.05), with no change in the synthesis of RANK and osteoprotegerin (p> 0.05). Interestingly, in the exposed animals, an early increase in the synthesis of osteocalcin, bone sialoprotein and osteopontin was also identified at 3 days of exposure (p <0.05), not sustained over time (p> 0.05). Cigarette smoke modulates osteogenic and osteoclastogenic signaling in the middle palatal suture of young rats, although morphological changes have not been evidenced. (AU)

• Processo FAPESP: 15/05180-2 - Análise das proteínas ósseas liberadas após a aplicação de forças ortopédicas em ratos fumantes passivos.
• Beneficiário: Maria Bernadete Sasso Stuani
• Modalidade de apoio: Auxílio à Pesquisa - Regular