Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface

Autor(es)

Diana Gabriela Soares ^[1] ; Nancy Tomoko Sacono ^[2] ; Ana Paula Dias Ribeiro ^[3] ; Maria Luisa Leite ^[4] ; Carla Caroline de Oliveira Duque ^[5] ; Marjorie de Oliveira Gallinari ^[6] ; Leandro Edgar Pacheco ^[7] ; Josimeri Hebling ^[8] ; Carlos Alberto de Souza Costa ^[9]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Endodontics and Dental Materials, Bauru School of Dentistry. Department of Operative Dentistry - Brasil

^[2] Universidade Paulista. Department of Pediatric Dentistry - Brasil

^[3] University of Florida. College of Dentistry. Department of Restorative Dental Sciences - Estados Unidos

^[4] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil

^[5] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil

^[6] University of São Paulo. Endodontics and Dental Materials, Bauru School of Dentistry. Department of Operative Dentistry - Brasil

^[7] University of São Paulo. Endodontics and Dental Materials, Bauru School of Dentistry. Department of Operative Dentistry - Brasil

^[8] São Paulo State University. Araraquara School of Dentistry. Department of Orthodontics and Pediatric Dentistry - Brasil

^[9] São Paulo State University. School of Dentistry. Department of Physiology and Pathology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 33, n. 2, p. 83-90, 2022-04-29.

Resumo

Abstract This paper aimed to assess the influence of adhesive restoration interface on the diffusion of hydrogen peroxide (H2O2), indirect toxicity, and pro-inflammatory mediators expression by odontoblast-like cells, after in-office tooth whitening. Dental cavities prepared in bovine enamel/dentin discs were adhesively restored and subjected or not to hydrolytic degradation (HD). A whitening gel with 35% H2O2 (WG) was applied for 45 min onto restored and non-restored specimens adapted to artificial pulp chambers giving rise to the groups: SD- intact discs (control); SD/HP- whitened intact discs; RT/HP- restored and whitened discs; and RT/HD/HP- restored and whitened discs subjected to HD. The extracts (culture medium + WG components diffused through enamel/dentin/restoration interface) were collected and applied to odontoblast-like MDPC-23 cells. The study evaluated the amount of H2O2 in the extracts, as well as the cell viability (CV), cell morphology (CM), and gene expression of inflammatory mediators (TNF-α and COX-2) by the pulp cells exposed to the extracts (ANOVA and Tukey tests; 5% significance). All whitened groups presented lower CV than SD (control; p<0.05). The highest CV reduction and gene expression of TNF-α and COX-2 was observed in the RT/HD/HP group in comparison with SD/HP and RT/HP (control; p<0.05). CM alterations occurred in all whitened groups. The intensity of these cell side effects was directly related with the amount of H2O2 in the extracts. We concluded that adhesive restoration of dental cavity increases the H2O2 diffusion after in-office whitening, enhancing the indirect toxicity of this therapy and trigger pro-inflammatory overexpression by MDPC-23 cells. (AU)

• Processo FAPESP: 20/09095-8 - Desenvolvimento de estratégias inovadoras para clareamento dental de consultório: eficácia estética e biocompatibilidade associadas à redução do tempo clínico
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular