Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples

Autor(es)

Amaral, Alan Goncalves ; Moretto, Isabela Aparecida ; Zandonadi, Flavia da Silva ; Zamora-Obando, Hans Rolando ; Rocha, Isabela ; Sussulini, Alessandra ; de Thomaz, Andre Alexandre ; Oliveira, Regina Vincenzi ; dos Santos, Aline Mara ; Simionato, Ana Valeria Colnaghi

• Tipo de documento: Artigo Científico
• Fonte: RONTIERS IN CHEMISTR; v. 10, p. 13-pg., 2022-04-08.

Resumo

Cardiovascular diseases (CVDs) are noncommunicable diseases known for their complex etiology and high mortality rate. Oxidative stress (OS), a condition in which the release of free radical exceeds endogenous antioxidant capacity, is pivotal in CVC, such as myocardial infarction, ischemia/reperfusion, and heart failure. Due to the lack of information about the implications of OS on cardiovascular conditions, several methodologies have been applied to investigate the causes and consequences, and to find new ways of diagnosis and treatment as well. In the present study, cardiac dysfunction was evaluated by analyzing cells' alterations with untargeted metabolomics, after simulation of an oxidative stress condition using hydrogen peroxide (H2O2) in H9c2 myocytes. Optimizations of H2O2 concentration, cell exposure, and cell recovery times were performed through MTT assays. Intracellular metabolites were analyzed right after the oxidative stress (oxidative stress group) and after 48 h of cell recovery (recovery group) by ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) in positive and negative ESI ionization mode. Significant alterations were found in pathways such as "alanine, aspartate and glutamate metabolism", "glycolysis", and "glutathione metabolism", mostly with increased metabolites (upregulated). Furthermore, our results indicated that the LC-MS method is effective for studying metabolism in cardiomyocytes and generated excellent fit ((RY)-Y-2 > 0.987) and predictability (Q(2) > 0.84) values. (AU)

• Processo FAPESP: 18/07383-6 - Sinalização pela quinase de adesão focal em miócitos cardíacos: novas interações proteicas e funções celulares
• Beneficiário: Aline Mara dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/05965-8 - Estratégias metabolômicas baseadas em LC-HRMS para investigação de potenciais biomarcadores para diagnóstico clínico - Fase I
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• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50244-6 - UHPLC acoplado a espectrômetro de massa do tipo híbrido com analisador quadrupolar e time of flight
• Beneficiário: Quezia Bezerra Cass
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários