Biofilm accumulation and sucrose rinse modulate calcium and fluoride bioavailability in the saliva of children with early childhood caries

Autor(es)

Crescente, Camila Lopes ; de Sousa, Emerson Tavares ; Lima-Holanda, Aline Tavares ; Steiner-Oliveira, Carolina ; Nobre-dos-Santos, Marines

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1, p. 10-pg., 2022-06-18.

Resumo

This study aimed at investigating the combined effect of biofilm accumulation and 20% sucrose rinse on the modulation of calcium (Ca2+), phosphate (P-i), and fluoride (F-) bioavailability in the saliva of children with early childhood caries (ECC). Fifty-six preschoolers of both genders were evaluated according to caries experience and activity: caries-free (CF, n = 28) and with ECC (n = 28) and then, submitted to biofilm intervention (biofilm accumulation). In each situation, saliva samples were collected before and five minutes after a 20% sucrose rinse to determine the concentrations of Ca2+, P-i, and F-. Calcium concentration was significantly lower in the biofilm accumulation situation compared to the situation of biofilm mechanical control (p <= 0.01), except for CF children after sucrose rinse. Biofilm accumulation increased salivary calcium concentration in children with ECC after sucrose rinse (p = 0.04), whereas mechanical biofilm control reduced it in both groups (p = 0.000). Phosphate concentration was influenced by mechanical control of biofilm in CF children (p = 0.03). The fluoride bioavailability was reduced by sucrose rinse and biofilm accumulation in CF and ECC children (p <= 0.002). In conclusion, the combined effect of biofilm accumulation and sucrose rinse modifies the bioavailability of calcium and fluoride in the saliva of children with early childhood caries. (AU)

• Processo FAPESP: 17/17630-8 - Análise da atividade da anidrase carbônica VI e ±-amilase na saliva e no biofilme após desafio cariogênico com sacarose e amido em crianças de 3 a 5 anos
• Beneficiário: Marines Nobre dos Santos Uchôa
• Modalidade de apoio: Auxílio à Pesquisa - Regular