Screen-Printed Electrodes on Tyvek Substrate as Low-Cost Device to Applications in Alzheimer's Disease Detection

Autor(es)

Palley, Bianca Fortes ; Artur, Julio Cesar ; de Arruda, Milena Nakagawa ; de Souza, Gustavo Freitas ; Graves, David Alexandro ; de Carvalho Bovolato, Ana Livia ; Deffune, Elenice ; Schelp, Arthur Oscar ; Goncalves, Emerson Sarmento ; de Moraes, Marli Leite

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Electrochemical Society; v. 169, n. 3, p. 7-pg., 2022-03-01.

Resumo

Alzheimer' s disease (AD) is a progressive neurodegenerative disease characterized by impairment of cognitive functions and memory deterioration, which requires an early diagnosis for effective treatment. The use of immunosensors to detect Alzheimer's disease biomarkers stand out as a quick and cheap alternative for early detection of the disease. The development of Screen-Printed Electrodes (SPEs) meets a growing demand in the market for applications such as signal transducers in biosensor devices. In this work was development a method for fabrication of low cost SPE for application in the detection of Alzheimer' s disease through autoantibodies. It was produced microelectrodes on polyvinyl chloride (PVC), polyvinylidene fluoride (PVDF) and high-density polyethylene (HDPE) manufactured by DuPont Tyvek (R) substrates. SPEs produced on Tyvek substrates have shown promising results for low-cost, disposable and flexible sensors. The carbon paste showed excellent adhesion to Tyvek (R) substrate and the electrodes produced showed an electrochemical performance comparable to commercial electrodes, besides reproducible. The detection results showed that it is possible to detect anti-A beta 40 autoantibodies in real samples of serum and cerebrospinal fluid using the electrode modified with two bilayers of (PEI/(DDPG + A beta 40)) since the capacitance increased more in positive sample with the presence of autoantibody than in negative samples. (C) 2022 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited. (AU)

• Processo FAPESP: 17/17661-0 - Obtenção de óxido de grafeno reduzido para aplicação em sistemas de sensores da indústria aeroespacial
• Beneficiário: Emerson Sarmento Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/27394-5 - Acumuladores de carbono poroso de alto desempenho para suporte energético de hubs complexos alimentados por sistema de dessalinização de águas
• Beneficiário: Emerson Sarmento Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/04427-5 - Obtenção e caracterização de eletrodos carbonosos nanoestruturados para processos de deionização capacitiva
• Beneficiário: David Alexandro Graves
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 18/04648-9 - Imunossensor para o diagnóstico da Doença de Alzheimer no estágio pré-clínico através da detecção de autoanticorpos
• Beneficiário: Marli Leite de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Regular