Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

Autor(es)

Zorn, Savienne ; Carvalho, Ana ; Bento, Heitor ; Gambarato, Bruno ; Pedro, Guilherme ; da Silva, Ana ; Goncalves, Rhyan ; Da Ros, Patricia ; Silva, Messias

• Tipo de documento: Artigo Científico
• Fonte: MEMBRANES; v. 12, n. 3, p. 15-pg., 2022-03-01.

Resumo

In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of Scenedesmus obliquus adhering to the mycelium of filamentous fungal Mucor circinelloides. This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores' incubation, a culture medium containing only 2 g center dot L-1 of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells' incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 20/15513-7 - Scale up da produção de L-asparaginase empregando microrganismo geneticamente modificado
• Beneficiário: Heitor Buzetti Simões Bento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/01386-3 - Liquens artificiais e a integração de produtos de alto valor agregado a uma biorrefinaria
• Beneficiário: Savienne Maria Fiorentini Elerbrock Zorn
• Modalidade de apoio: Bolsas no Brasil - Doutorado