rumAB genes from SXT/R391 ICEs confer UV-induced mutability to Proteus mirabilis hosts and improve conjugation after UV irradiation

Autor(es)

Sato, Juliana Lumi ; de Holanda Fonseca, Douglas Lyra ; Galhardo, Rodrigo S.

• Tipo de documento: Artigo Científico
• Fonte: DNA Repair; v. 112, p. 10-pg., 2022-04-01.

Resumo

Proteus mirabilis is one of the Enterobacteriales species that has been deemed as non-mutable by DNA damaging agents. A genomic analysis of P. mirabilis genomes shows that this species often does not carry pol V-encoding genes in its chromosome, which are responsible for SOS mutagenesis in other bacteria. On the other hand, the highly active umuDC homologs rumAB are carried in the mobile integrative conjugative elements (ICEs) from the SXT/R391 family that are frequently found in this species. Here we show that isolates devoid of SXT/R391 are indeed weakly or non-mutable by UV-light exposure, in contrast to isolates carrying SXT/R391 elements, some of which are mutable under these conditions. SXT/R391-bearing isolates display a variable behavior regarding UV induced mutagenesis, despite the functionality of rumAB homologs carried by them. We also show that the globally dispersed ICEPmiJpn1 confers UV mutability to otherwise non-mutable isolates and demonstrate that this phenomenon is dependent on rumAB genes. Finally, we investigated whether rumAB genes could play a role in the conjugation of ICEPmiJpn1 and found that these genes improve the conjugation of SXT/R391 by a small margin. Taken together, these results show that the presence of rumAB in SXT/R391 ICEs endows the hosts with damage-inducible mutagenesis ability and promotes a small but significant enhancement in element transfer after exposure to UV light. (AU)

• Processo FAPESP: 18/23872-7 - Elementos conjugativos integrativos da família SXT/R391 em Proteus mirabilis e a sua relação com resistência, mutagênese e conjugação
• Beneficiário: Juliana Lumi Sato
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 20/00535-5 - Influência de elementos integrativos e conjugativos (ICEs) da família SXT/R391 em aspectos fisiológicos e patogênicos de Proteus Mirabilis
• Beneficiário: Douglas Lyra de Holanda Fonseca
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/19435-3 - Papel de danos no DNA e função mitocondrial em envelhecimento vascular, imune e neurológico (DNA MoVINg)
• Beneficiário: Carlos Frederico Martins Menck
• Modalidade de apoio: Auxílio à Pesquisa - Temático