Propranolol inhibits cell viability and expression of the pro-tumorigenic proteins Akt, NF-KB, and VEGF in oral squamous cell carcinoma

Autor(es)

Shibuya, Caroline Miho ; Tjioe, Kellen Cristine ; Penha Oliveira, Sandra Helena ; Bernabe, Daniel Galera

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 136, p. 9-pg., 2022-04-01.

Resumo

Background: Propranolol (PPL) has been suggested as an option for the treatment of various types of cancer. However, data regarding its effectiveness against oral cancer are scarce. Thus, we aimed to evaluate the antitumor potential of PPL in oral squamous cell carcinoma (OSCC) in vitro.Methods: OSCC cell lines, SCC-9, SCC-25, and Cal27, were treated with PPL at different times and concentrations. OSCC cells were treated with PPL alone or in combination with cisplatin (CDDP) or 5-fluorouracil (5-FU). Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of phosphorylated (p)-Akt, p-S6, p-PTEN, p-P65, and VEGF was verified by immunofluorescence. The migratory activity of OSCC cells was evaluated using a wound-healing assay.Results: PPL reduced OSCC cell viability in a dose-and time-dependent manner. Concentrations above 300 mu M, 110 mu M, and 100 mu M for SCC-9, Cal27, and SCC-25, respectively, significantly eliminated tumor cells. The combination of PPL with CDDP and 5-FU enhanced their antitumor effects. There was a modest difference between the use of the IC30 and IC50 of PPL in the combinatory options. PPL downregulated p-P65 NF-KB and VEGF expression in SCC-9 and Cal27 cells but not in SCC-25 cells. PPL inhibited the phosphorylation of Akt and s6 and increased the phosphorylation of PTEN in all OSCC cell lines studied. PPL inhibited OSCC cell migration after 24 h of treatment.Conclusion: PPL was effective against oral cancer cells and enhanced standard-of-care. PPL inhibited cell viability and the expression of pAkt, NF-KB, and VEGF. (AU)

• Processo FAPESP: 16/25255-0 - Efeitos do estresse e seus mediadores sobre a carcinogênese e progressão do câncer de cabeça e pescoço
• Beneficiário: Daniel Galera Bernabé
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/00051-2 - Influência dos hormônios do estresse sobre a resistência das células do câncer de boca ao tratamento quimioterápico
• Beneficiário: Kellen Cristine Tjioe
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/18650-2 - Efeito da ação anti-tumoral do propranolol sobre linhagens de células de câncer de boca
• Beneficiário: Caroline Miho Shibuya
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica