Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis

Autor(es)

Sato, Mariana Rillo ; Oshiro-Junior, Joao Augusto ; Rodero, Camila Fernanda ; Boni, Fernanda Isadora ; Sousa Araujo, Victor Hugo ; Bauab, Tais Maria ; Nicholas, Dean ; Callan, John F. ; Chorilli, Marlus

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL DE MYCOLOGIE MEDICALE; v. 32, n. 4, p. 8-pg., 2022-11-01.

Resumo

Introduction and Aim: The indiscriminate use and adverse effects of the main conventional antifungal agents compromise the effectiveness of treating vulvovaginal candidiasis (VVC), mainly caused by the species Candida albicans. This study evaluated the effectiveness of photodynamic therapy (PDT) and the in vitro and in vivo anti-candida potential of the hypericin (HYP)-loaded nanostructured lipid carriers (NLC). Materials and Methods: Empty NLC and NLC-HYP were characterized by the dynamic light scattering technique and transmission electron microscopy to evaluate the average particle size distribution and its morphologies. The in vitro inhibition photodynamic effect of the systems was tested to reduce the planktonic viability of C. albicans. The therapeutic assay photodynamic of the systems was performed to treat VVC in mice. Results: Empty NLC and NLC-HYP presented values of average hydrodynamic diameter, polydispersity index, and z-potential from 136 to 133 nm, 0.16 to 0.22, and -18 to-30 mV, respectively, on day 30. Microscopically, the systems showed spherical morphologies and nanoscale particles. Furthermore, in the in vitro inhibition assay, the treatment of PDT with NLC-HYP (NLC-HYP+) showed a significant reduction of the C. albicans planktonic viability compared to YNB negative control after 5 min of LED light irradiation. In the in vivo therapeutic assay, the antifungal group (vaginal antifungal cream) and NLC-HYP+ evaluated in the dark and by PDT, respectively, had a significant log10 reduction in fungal burden compared to the infected group on day 8 of the VVC treatment. Conclusion: Due to the in vitro and in vivo anti-candida potential, PDT-mediated systems can be an effective strategy in VVC therapy. Published by Elsevier Masson SAS on behalf of SFMM. (AU)

• Processo FAPESP: 16/11198-4 - Carreadores lipídicos nanoestruturados dispersos em hidrogéis de geleificação in situ para administração vaginal de hipericina associados à terapia fotodinâmica no tratamento da candidíase vulvovaginal
• Beneficiário: Mariana Rillo Sato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/17573-7 - Potencial efeito da hipericina incorporada em sistemas nanoestruturados mucoadesivos associados as terapias fotodinâmicas e sonodinâmicas antimicrobianas contra cepas de Candida albicans
• Beneficiário: Mariana Rillo Sato
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/10261-2 - Avaliação do potencial de carreadores lipídicos nanoestruturados para co-encapsulação de curcumina e fluconazol dispersos em hidrogéis termorreponsivos no tratamento de candidíase vulvovaginal
• Beneficiário: Victor Hugo Sousa Araujo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/17913-5 - Desenvolvimento farmacotécnico e atividade anti-Candida albicans in vitro e in vivo de carreadores lipídicos nanoestruturados dispersos em hidrogéis para administração vaginal de hipericina
• Beneficiário: Marlus Chorilli
• Modalidade de apoio: Auxílio à Pesquisa - Regular