Characterization of the Neospora caninum peroxiredoxin: a novel peroxidase and antioxidant enzyme

Autor(es)

Venancio-Brochi, Jade Cabestre ; Pereira, Luiz Miguel ; Baroni, Luciana ; Abreu-Filho, Pericles Gama ; Yatsuda, Ana Patricia

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 121, n. 6, p. 14-pg., 2022-04-01.

Resumo

Neospora caninum, an apicomplexan parasite, is the etiological agent of neosporosis, a disease that leads to neurological symptoms in dogs and abortion in cattle. Vaccine or drug treatments for neosporosis remain to be determined. Therefore, it is of undeniable relevance to investigate new molecules involved in the parasite's successful survival within the host cell. The aim of this study was to characterize the N. caninum peroxiredoxin (NcPrx), an enzyme involved in the redox system of the parasite. The NcPrx amino acid sequence showed high identity and similarity compared to homologues representatives of Apicomplexa phylum. The recombinant NcPrx (rNcPrx) was cloned and expressed in Escherichia coli (BL21) with the predicted molecular weight (22 kDa), and the identity of monomer and dimer forms of rNcPrx was confirmed by mass spectrometry. Native and recombinant NcPrx were detected by ELISA and western blot, using the polyclonal anti-rNcPrx serum. Multiphoton analysis showed that NcPrx is localized in tachyzoite cytosol. H2O2 treatment increased the rNcPrx dimerization in vitro, and associated with the in silico data, we suggest that NcPrx belongs to typical 2-Cys Prx group (AhpC/Prx1 family). rNcPrx also increased the H2O2 clearance and protected plasmidial DNA under oxidative conditions. Finally, H2O2 increased the NcPrx dimerization in intracellular and extracellular tachyzoites suggesting that it is enrolled in H2O2 clearance and sensing in N. caninum. (AU)

• Processo FAPESP: 18/21020-3 - Fator de despolimerização de actina (NcADF): oxidação como regulador da atividade de NcADF na morte celular programada de Neospora caninum
• Beneficiário: Ana Patricia Yatsuda Natsui
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/05758-5 - Controle redox em Neospora caninum: investigação de enzimas do sistema antioxidante
• Beneficiário: Jade Cabestre Venancio Brochi
• Modalidade de apoio: Bolsas no Brasil - Doutorado