Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production

Autor(es)

Luciane Dias de Oliveira ^[1] ; Felipe Eduardo de Oliveira ^[2] ; Bárbara Araujo Hatje ^[3] ; Marcia Carneiro Valera ^[4] ; Cláudio Antonio Talge Carvalho ^[5] ; Amjad Abu Hasna ^[6]

Afiliação do(s) autor(es)

^[1] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil

^[2] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil

^[3] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil

^[4] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil

^[5] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil

^[6] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 33, n. 6, p. 36-43, 2022-12-05.

Resumo

Abstract The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single-rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide. (AU)

• Processo FAPESP: 11/23202-2 - Eficácia da instrumentação rotatória utilizando diferentes substâncias químicas auxiliares em neutralizar os efeitos citotóxicos do ácido lipoteicóico (LTA) de Enterococcus faecalis em canais radiculares
• Beneficiário: Luciane Dias de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular