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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production

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Author(s):
Luciane Dias de Oliveira [1] ; Felipe Eduardo de Oliveira [2] ; Bárbara Araujo Hatje [3] ; Marcia Carneiro Valera [4] ; Cláudio Antonio Talge Carvalho [5] ; Amjad Abu Hasna [6]
Total Authors: 6
Affiliation:
[1] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil
[2] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil
[3] São Paulo State University. Institute of Science and Technology. Department of Bioscience and Oral Diagnosis - Brasil
[4] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil
[5] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil
[6] São Paulo State University. Endodontics division, Institute of Science and Technology. Department of Restorative Dentistry - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Dental Journal; v. 33, n. 6, p. 36-43, 2022-12-05.
Abstract

Abstract The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single-rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide. (AU)

FAPESP's process: 11/23202-2 - Efficacy of instrumentation rotary with different auxiliary chemical substances in neutralizing the toxic effects of lipotheicoic acid (LTA) from Enterococcus faecalis in root canals
Grantee:Luciane Dias de Oliveira
Support Opportunities: Regular Research Grants