Models for cytotoxicity screening of antileishmanial drugs: what has been done so far?

Autor(es)

Brioschi, Mariana B. C. ; Coser, Elizabeth M. ; Coelho, Adriano C. ; Gadelha, Fernanda R. ; Miguel, Danilo C.

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS; v. 60, n. 2, p. 11-pg., 2022-08-01.

Resumo

A growing number of studies have demonstrated the in vitro potential of an impressive number of antileishmanial candidates in the past years. However, the lack of uniformity regarding the choice of cell types for cytotoxicity assays may lead to uncomparable and inconclusive data. In vitro assays relying solely on non-phagocytic cell models may not represent a realistic result as the effect of an antileishmanial agent should ideally be presented based on its cytotoxicity profile against reticuloendothelial system cells. In the present review, we have assembled studies published in the scientific literature from 2015 to 2021 that explored leishmanicidal candidates, emphasising the main host cell models used for cytotoxicity assays. The pros and cons of different host cell types as well as primary cells and cell lines are discussed in order to draw attention to the need to establish standardised protocols for preclinical testing when assessing new antileishmanial candidates. (C) 2022 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved. (AU)

• Processo FAPESP: 14/21129-4 - O papel das proteínas ligantes de ácidos graxos na infecção de macrófagos por Leishmania: um alvo potencial para novas drogas contra leishmaniose
• Beneficiário: Danilo Ciccone Miguel
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/21171-6 - Paromomicina no tratamento da Leishmaniose Tegumentar: investigação in vitro, in vivo e na identificação de marcadores moleculares associados à suscetibilidade e resistência
• Beneficiário: Adriano Cappellazzo Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/22175-3 - Identificação e validação funcional de genes associados à suscetibilidade e resistência à paromomicina em Leishmania spp
• Beneficiário: Elizabeth Magiolo Coser
• Modalidade de apoio: Bolsas no Brasil - Doutorado