Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway

Autor(es)

Cardoso, Luana Pereira ; de Sousa, Stefanie Oliveira ; Gusson-Zanetoni, Juliana Prado ; de Melo Moreira Silva, Laura Luciana de Melo Moreira ; Frigieri, Barbara Maria ; Henrique, Tiago ; Tajara, Eloiza Helena ; Oliani, Sonia Maria ; Rodrigues-Lisoni, Flavia Cristina

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICALS; v. 16, n. 1, p. 21-pg., 2023-01-01.

Resumo

Cervical cancer is the fourth-most common type of cancer in the world that causes death in women. It is mainly caused by persistent infection by human papillomavirus (HPV) that triggers a chronic inflammatory process. Therefore, the use of anti-inflammatory drugs is a potential treatment option. The effects of piperine, an amino alkaloid derived from Piper nigrum, are poorly understood in cervical cancer inflammation, making it a target of research. This work aimed to investigate the antitumor effect of piperine on cervical cancer and to determine whether this effect is modulated by the cyclooxygenase 2 (PTGS2) pathway using in vitro model of cervical cancer (HeLa, SiHa, CaSki), and non-tumoral (HaCaT) cell lines. The results showed that piperine reduces in vitro parameters associated with neoplastic evolution such as proliferation, viability and migration by cell cycle arrest in the G1/G0 and G2/M phases, with subsequent induction of apoptosis. This action was modulated by downregulation of cyclooxygenase 2 (PTGS2) pathway, which in turn regulates the secretion of cytokines and the expression of mitogen-activated protein kinases (MAPKs), metalloproteinases (MMPs), and their antagonists (TIMPs). These findings indicate the phytotherapeutic potential of piperine as complementary treatment in cervical cancer. (AU)

• Processo FAPESP: 17/02100-3 - Piper cubeba: aplicação em diferentes linhagens de células tumorigênicas
• Beneficiário: Flávia Cristina Rodrigues Lisoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/19949-7 - Avaliação da atividade imunomoduladora da proteína anexina A1 em lesões cutâneas e placentárias resultantes do Diabetes Mellitus
• Beneficiário: Sonia Maria Oliani
• Modalidade de apoio: Auxílio à Pesquisa - Regular