A chromosomal-scale reference genome of the New World Screwworm, Cochliomyia hominivorax

Autor(es)

Tandonnet, Sophie ; Krsticevic, Flavia ; Basika, Tatiana ; Papathanos, Philippos A. ; Torres, Tatiana T. ; Scott, Maxwell J.

• Tipo de documento: Artigo Científico
• Fonte: DNA Research; v. 30, n. 1, p. 9-pg., 2023-02-01.

Resumo

The New World Screwworm, Cochliomyia hominivorax (Calliphoridae), is the most important myiasis-causing species in America. Screwworm myiasis is a zoonosis that can cause severe lesions in livestock, domesticated and wild animals, and occasionally in people. Beyond the sanitary problems associated with this species, these infestations negatively impact economic sectors, such as the cattle industry. Here, we present a chromosome-scale assembly of C. hominivorax's genome, organized in 6 chromosome-length and 515 unplaced scaffolds spanning 534 Mb. There was a clear correspondence between the D. melanogaster linkage groups A-E and the chromosomal-scale scaffolds. Chromosome quotient (CQ) analysis identified a single scaffold from the X chromosome that contains most of the orthologs of genes that are on the D. melanogaster fourth chromosome (linkage group F or dot chromosome). CQ analysis also identified potential X and Y unplaced scaffolds and genes. Y-linkage for selected regions was confirmed by PCR with male and female DNA. Some of the long chromosome-scale scaffolds include Y-linked sequences, suggesting misassembly of these regions. These resources will provide a basis for future studies aiming at understanding the biology and evolution of this devastating obligate parasite. (AU)

• Processo FAPESP: 19/10966-6 - Evolução do parasitismo em três dimensões: filogenética, fenotípica e genômica
• Beneficiário: Tatiana Teixeira Torres
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/05636-4 - Dimensions US-BIOTA-São Paulo: além da aparência: compreendendo as origens evolutivas e genéticas de diversas especializações tróficas em moscas varejeiras
• Beneficiário: Tatiana Teixeira Torres
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 19/07285-7 - Evolução de traços complexos: o exemplo do hábito alimentar em Calliphoridae
• Beneficiário: Sophie Hokulani Véronique Jessica Tandonnet
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado