MALDI-TOF Mass Spectrometry for the Diagnosis of Citrus Canker Caused by Xanthomonas citri subsp. citri

Autor(es)

Niculau, Edenilson dos Santos ; Ferreira, Douglas ; Rodrigues-Filho, Edson ; Behlau, Franklin ; Magnani, Rodrigo Facchini ; Toffano, Leonardo ; Prieto, Evandro Luis ; Fernandes, Joao Batista ; da Silva, Maria Fatima das Gracas Fernandes

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 27, n. 24, p. 14-pg., 2022-12-01.

Resumo

Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a disease that causes serious problems to the global citrus industry. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS) has been used in human medicine to diagnose various diseases caused by both fungi and bacteria. In agriculture, this technique has potential for the diagnosis of diseases due to the low cost of large-scale analysis and quickness. This study showed that MALDI-TOF MS combined with chemometric analysis was effective for differentiating the macromolecule profile of orange leaves with canker lesions, healthy leaves, and leaves with phytotoxicity symptoms, proving that this technique may be used for the rapid diagnosis of citrus canker. (AU)

• Processo FAPESP: 08/57859-5 - Controle biorracional de insetos pragas
• Beneficiário: Maria Fátima das Graças Fernandes da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/22975-8 - Estudo químico de Xanthomonas axonopodis pv. citri e sua influência no perfil químico de Citrus sinensis
• Beneficiário: Edenilson dos Santos Niculau
• Modalidade de apoio: Bolsas no Brasil - Doutorado