Amperometric Biosensor Based on Laccase Enzyme, Gold Nanoparticles, and Glutaraldehyde for the Determination of Dopamine in Biological and Environmental Samples

Autor(es)

Santos, Anderson M. ; Wong, Ademar ; Fatibello-Filho, Orlando ; Moraes, Fernando C.

• Tipo de documento: Artigo Científico
• Fonte: C-JOURNAL OF CARBON RESEARCH; v. 8, n. 3, p. 13-pg., 2022-09-01.

Resumo

The present work reports the development and application of an amperometric biosensor based on carbon paste electrode modified with laccase enzyme, glutaraldehyde, and gold nanopartides (Lac-Glu-AuNPs/CPE) for the determination of the neurotransmitter dopamine (DA). The materials were characterized morphologically and chemically using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry. Optimization studies were performed in order to determine the optimal amount of enzyme and pH level that can yield the best conditions of analysis. The application of the biosensor in optimal conditions using the amperometric technique yielded a linear concentration range of 8.0 x 10(-7) -6.2 x 10(-5) mol L-1 with a limit of detection of 6.0 x 10(-8) mol L-1. The proposed biosensor was successfully applied for the determination of DA in biological and environmental samples. In addition, the application of the biosensor for the conduct of electrochemical measurements showed that the sensing device has good repeatability and stability, and it does not suffer from matrix interference effects. The proposed biosensor exhibited an analytical signal of 85% after 10 days of consecutive use. (AU)

• Processo FAPESP: 17/10118-0 - Estudo e aplicação da tecnologia eletroquímica para a análise e a degradação de interferentes endócrinos: materiais, sensores, processos e divulgação científica
• Beneficiário: Marcos Roberto de Vasconcelos Lanza
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/01050-5 - Desenvolvimento e aplicação de sensores eletroquímicos e fotoeletroquímicos para a determinação de contaminantes emergentes em amostras de águas naturais
• Beneficiário: Orlando Fatibello Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/05454-9 - Desenvolvimento de sensores eletroquímicos descartáveis e fotoanodos fabricados com materiais semicondutores nanoestruturados para a determinação e degradação de disruptores endócrinos
• Beneficiário: Fernando Cruz de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Regular