In vitro antibacterial activity of green tea-loaded chitosan nanoparticles on caries-related microorganisms and dentin after Er:YAG laser caries removal

Autor(es)

Curylofo-Zotti, Fabiana Almeida ; Oliveira, Viviane De Cassia ; Marchesin, Analu Rodriguez ; Borges, Hiago Salge ; Tedesco, Antonio Claudio ; Corona, Silmara Aparecida Milori

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 38, n. 1, p. 8-pg., 2023-01-23.

Resumo

This study aimed to determine the inhibitory effects of green tea (Gt), EGCG, and nanoformulations containing chitosan (Nchi) and chitosan+green tea (Nchi+Gt) against Streptococcus mutans and Lactobacillus casei. In addition, the antibacterial effect of nanoformulations was evaluated directly on dentin after the selective removal of carious lesion. At first, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. mutans and L. casei isolates were investigated. In parallel, dentin specimens were exposed to S. mutans to induce carious lesions. Soft dentin was selectively removed by Er:YAG laser (n=33) or bur (n=33). Remaining dentin was biomodified with Nchi (n=11) or Gt+Nchi (n=11). Control group (n=11) did not receive any treatment. Dentin scraps were collected at three time points. Microbiological analyses were conducted and evaluated by agar plate counts. Gt at 1:32 dilution inhibited S. mutans growth while 1:16 was efficient against L. casei. EGCG at 1:4 dilution completely inhibited S. mutans and L. casei growth. Independently of the association with Gt, Nchi completely inhibited S. mutans at 1:4 dilution. For L. casei, different concentrations of Nchi (1:32) and Nchi+Gt (1:8) were required to inhibit cell growth. After selective carious removal, viability of S. mutans decreased (p < 0.001), without difference between bur and Er:YAG laser (p > 0.05). Treatment with Nchi and Nchi+Gt did not influence the microbial load of S. mutans on dentin (p > 0.05). Although variations in concentrations were noticed, all compounds showed antibacterial activity against S. mutans and L. casei. Both bur and Er:YAG laser have effectively removed soft dentin and reduced S. mutans counts. Nanoformulations did not promote any additional antibacterial effect in the remaining dentin. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/23862-1 - Avaliação da composição química da dentina afetada após remoção seletiva da lesão de cárie com Laser de Er:YAG e biomodificação com Camellia sinensis encapsulada em nanopartículas de quitosana
• Beneficiário: Analu Rodriguez Marchesin
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/11582-1 - Biomodificação da dentina com nanopartículas de quitosana fosforilada (NanoQF) após remoção seletiva da lesão de cárie com laser de Er:YAG: Avaliação das propriedades antimicrobianas e físico-químicas.
• Beneficiário: Fabiana Almeida Curylofo Zotti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/00720-4 - Influência da quitosana na reumidificação da dentina após remoção seletiva da lesão de cárie utilizando laser de Er:YAG. Análise longitudinal clínica, fotográfica, adaptação marginal da réplica da restauração e microbiológica
• Beneficiário: Silmara Aparecida Milori Corona
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/04807-2 - Função das proantocianidinas como nucleadores de biomineralização da dentina
• Beneficiário: Fabiana Almeida Curylofo Zotti
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado