Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation

Autor(es)

Vieira, Willians Fernando ; Malange, Kaue Franco ; de Magalhaes, Silviane Fernandes ; Paes Lemes, Julia Borges ; dos Santos, Gilson Goncalves ; Nishijima, Catarine Massucato ; Rodrigues de Oliveira, Alexandre Leite ; da Cruz-Hofling, Maria Alice ; Parada, Carlos Amilcar ; Tambeli, Claudia Herrera

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1, p. 18-pg., 2022-10-06.

Resumo

Several recent studies have established the efficacy of photobiomodulation therapy (PBMT) in painful clinical conditions. Diabetic neuropathy (DN) can be related to activating mitogen-activated protein kinases (MAPK), such as p38, in the peripheral nerve. MAPK pathway is activated in response to extracellular stimuli, including interleukins TNF-alpha and IL-1 beta. We verified the pain relief potential of PBMT in streptozotocin (STZ)-induced diabetic neuropathic rats and its influence on the MAPK pathway regulation and calcium (Ca2+) dynamics. We then observed that PBMT applied to the L4-L5 dorsal root ganglion (DRG) region reduced the intensity of hyperalgesia, decreased TNF-alpha and IL-1 beta levels, and p38-MAPK mRNA expression in DRG of diabetic neuropathic rats. DN induced the activation of phosphorylated p38 (p-38) MAPK co-localized with TRPV1(+) neurons; PBMT partially prevented p-38 activation. DN was related to an increase of p38-MAPK expression due to proinflammatory interleukins, and the PBMT (904 nm) treatment counteracted this condition. Also, the sensitization of DRG neurons by the hyperglycemic condition demonstrated during the Ca2+ dynamics was reduced by PBMT, contributing to its anti-hyperalgesic effects. (AU)

• Processo FAPESP: 18/05108-8 - Influência do laser de baixa intensidade nos neurônios: um estudo eletrofisiológico para ajudar a compreender os efeitos analgésicos do infravermelho na neuropatia diabética
• Beneficiário: Willians Fernando Vieira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/12673-5 - Efeitos da terapia laser de baixa intensidade (LLLT) em modelo de neuropatia diabética experimental
• Beneficiário: Willians Fernando Vieira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/25153-7 - Análise de expressão gênica diferencial de células do gânglio da raiz dorsal em modelo de diabetes e neuropatia diabética periférica
• Beneficiário: Carlos Amilcar Parada
• Modalidade de apoio: Auxílio à Pesquisa - Regular