Differential expression of regulators of the canonical Wnt pathway during the compensatory beta-cell hyperplasia in prediabetic mice

Autor(es)

Maschio, Daniela Aparecida ; Pinto Hernandes, Leticia Helena ; Alvares, Lucia Elvira ; Marques-Souza, Henrique ; Collares-Buzato, Carla Beatriz

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 611, p. 7-pg., 2022-04-28.

Resumo

We previously reported that the canonical Wnt signaling pathway is activated during compensatory islet hyperplasia in prediabetic mice. Here, we aimed to expand our knowledge concerning the Wnt signaling partners and modulators involved in this process. We report here that Axin1, Axin2, and DACT1, inhibitors of the canonical Wnt signaling pathway, displayed no change in their expression, while GSK-3 beta, a multi-functional kinase that acts as a negative regulator of this pathway as well as affects insulin secretion/action, was up-regulated in hyperplastic islets of prediabetic mice. We also observed that COUP-TFII, a protein that acts positively on Wnt-target genes related to cell proliferation, displays a significant increase in gene expression and protein content and is highly immunolabeled in islet cell nuclei of prediabetic mice compared to control islets. These findings suggest that GSK-3b and COUP-TFII may play a role in beta-cell dysfunction and hyperplasia during type 2 prediabetes. (C) 2022 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/25442-1 - Papel da via de sinalização Wnt canônica e não canônica na função e proliferação da célula beta pancreática durante a pré-diabetes experimental
• Beneficiário: Carla Beatriz Collares Buzato
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/09602-0 - Caracterização da rede de regulação cardiogênica controlada por Coup-TFII a partir de células-tronco embrionárias
• Beneficiário: Henrique Marques Barbosa de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores